ArticleMolecular biology reports2025
A step-by-step approach to establishing an efficient genetic transformation protocol for Chlorella vulgaris using electroporation.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Quantitative Evaluation of NR Locus-Targeted Nuclear Transformation inJournal of microbiology and biotechnology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChlorella vulgaris, a unicellular green microalga, has shown diverse applications in biotechnology. Particularly in microalgae genetic engineering, the genetic transformation of C. vulgaris offers a sustainable and cost-effective approach to producing recombinant proteins. Despite this, the thick cell wall of C. vulgaris poses challenges for efficient genetic transformation. This current study presents a detailed electrotransformation protocol of C. vulgaris using the pCAMBIA1303 vector, with validated transgene stability across generations.
methodsThe electroporation procedure described here includes C. vulgaris cell preparation and electroporation parameters such as pulse voltage, capacitance, and resistance. Prior to the cultivation of the transformed cells in a selective BG11 medium, recovery conditions of the newly transformed cells in a regular BG11 medium were also described. Through optimization of these parameters, an improved transformation efficiency while maintaining cell viability was achieved. Stability of the transformed C. vulgaris was also assessed across five generations.
resultsPositive amplifications of the pCAMBIA1303 in the PCR and RT-PCR assays were indicative of the success of the transformation procedure of C. vulgaris. DNA sequencing was then performed to validate the identity of these amplicons. Furthermore, the stability assessment demonstrated persistent maintenance of transformed C. vulgaris cultures across five generations, as evidenced by reproducible PCR amplification.
conclusionThe transformation procedure established here provides a reliable and reproducible method for carrying out genetic modification on C. vulgaris to facilitate its potential use as a bio-factory in numerous biotechnological applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.