Evidence map›Paper›PMID 41159751›Full record

ArticleJournal of virology2025

Human organoids for Risk Group 4 virus research: a new frontier in investigating Nipah virus infection of the central nervous system.

Gabriella Worwa, Shuǐqìng Yú, Amanda M W Hischak, Julie P Tran, Jeremy J Bearss, John Bernbaum, Daniel B Woodburn, Bapi Pahar, Jillian Geiger, Louis M Huzella and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Gabriella WorwaIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.ORCID 0000-0001-7485-2204
Shuǐqìng YúIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.
Amanda M W HischakIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.
Julie P TranIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.
Jeremy J BearssIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.
John BernbaumIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.
Daniel B WoodburnIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.ORCID 0000-0003-4936-2742
Bapi PaharIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.
Jillian GeigerIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.
Louis M HuzellaIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.
Santiago Vidal FreireDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Ian CrozierClinical Monitoring Research Program Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.ORCID 0000-0003-2485-4011
César Muñoz-FontelaBernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.ORCID 0000-0002-7725-2586
Gustavo PalaciosDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.ORCID 0000-0001-5062-1938
Nicole C KleinstreuerDivision of Program Coordination, Planning, and Strategic Initiatives, National Institutes of Health, Bethesda, Maryland, USA.
Lina WiderspickBernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
Jens H KuhnIntegrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.ORCID 0000-0002-7800-6045

Funding

WORK ORDER 126643 B539 EXPAND IC SUITE75N91019D00024 · NIAID · LEIDOS BIOMEDICAL RESEARCH, INC. · PI BRISCOE, LYNN · 2019 to 2025
$3932.6M
NCI NIH HHS 75N91019D00024NIH HHS HHSN272201800013C
6 · The paper itself

Abstract

New approach methodologies, such as high-complexity IMPORTANCE: Advanced development of medical countermeasures against Risk Group 4 viruses, such as the Nipah virus, historically required testing in mammals under the FDA Animal Rule and translation of data to inform clinical trials in humans. Because the application of human organoids in research on viruses pathogenic for humans is conspecific, it bears the potential to reduce, refine, or replace animal studies where unnecessary. Human cerebral organoids are three-dimensional cell aggregates that resemble the developing human brain functionally and structurally. Brain organoids may be valuable in investigating the replication, neuroinvasion, pathogenesis, virulence, and persistence of neurotropic viruses and provide scientific discernment when developing medical countermeasures destined for the human end-user.

Indexed as

Animal Use AlternativesCentral Nervous System Viral DiseasesHenipavirus InfectionsNipah VirusOrganoidsHumanscentral nervous system infectioncomplex in vitro systemhuman cerebral organoidmaximum containmentNAMsnew approach methodologiesNipah virusNiV BangladeshNiV Malaysiareplication kinetics

Identifiers

PMID41159751
PMCPMC12645973

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.