ArticleJournal of clinical microbiology2025
A multiplex Mtb-specific FluoroSpot assay measuring IFNγ, IL-2, and TNF-secreting cells can improve accuracy and differentiation across the tuberculosis spectrum.
Elin Folkesson, Fariba Foroogh, Linn Kleberg, Vera Kjellgren, Mathilda Jakobsson, Lisann Grunewald, Jens Hellberg, Jennifer Ryberg, Zahra Maher, Carolina Sousa Silva and 7 more
Abstract readEvaluation Study
In one paragraphArticle in Journal of clinical microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 · The registryThe trial behind it
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5 · Who and what moneyAuthors and funding
17 authors.
Elin FolkessonDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0002-6585-6235 Fariba ForooghDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Linn KlebergDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.ORCID 0009-0004-3839-3281 Vera KjellgrenDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Mathilda JakobssonDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Lisann GrunewaldDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Jens HellbergDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Jennifer RybergDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Zahra MaherDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Carolina Sousa SilvaDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0003-2701-179X Maia S GowerDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0002-0045-6919 Margarida Correia-NevesDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Gunilla KälleniusDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0002-8921-0551 Judith Bruchfeld *Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0001-5399-0982 Christopher Sundling *Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0002-6138-690X Funding
Hjärt-Lungfonden 20180386Hjärt-Lungfonden 20200194Hjärt-Lungfonden 20220566Hjärt-Lungfonden 20230244Stiftelsen Läkare mot AIDS Forskningsfond Fob2022-0012Svenska Läkaresällskapet SLS-934363Svenska Läkaresällskapet SLS-960484Svenska Sällskapet för Medicinsk Forskning CG-24-0012-BSwedish Research Council 2019-04663Swedish Research Council 2020-03602Swedish Research Council 2021-03706Swedish Research Council 2023-01943
6 · The paper itselfAbstract
Tuberculosis (TB) remains a major global health challenge, with current diagnostic tools unable to reliably distinguish between individuals at different stages of the TB spectrum of infection (TBI) and disease (TBD). Interferon-gamma release assays (IGRAs), while commonly used, are limited by their reliance on a single cytokine and inability to differentiate disease states. In this study, we evaluated a multiplex FluoroSpot assay that simultaneously detects interferon gamma (IFNγ), interleukin (IL)2, and tumor necrosis factor (TNF)-secretion in response to
Indexed as
CytokinesInterferon-gammaInterferon-gamma Release TestsInterleukin-2Mycobacterium tuberculosisTuberculosisTumor Necrosis Factor-alphaAdolescentAdultAgedAntigens, BacterialBacterial ProteinsFemaleHumansLeukocytes, MononuclearMaleAntigens, BacterialBacterial ProteinsCytokinesESAT-6 protein, Mycobacterium tuberculosisInterferon-gammaInterleukin-2Tumor Necrosis Factor-alphacytokinesdiagnosticsFluoroSpotimmuno-assaymycobacteriumT cellstuberculosis
Identifiers
PMID41159744
PMCPMC12710326
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