Evidence map›Paper›PMID 41158609›Full record

ArticleChem & bio engineering2025

Semirational Protein Engineering Yields Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators with Enhanced Brightness and Red Shifted Absorption Bands.

Dmitrii M Nikolaev, Ekaterina M Metelkina, Nikita A Domskii, Ilya R Osadchy, Andrey S Mereshchenko, Stanislav A Bondarev, Galina A Zhouravleva, Andrey A Shtyrov, Maxim S Panov, Mikhail N Ryazantsev

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chem & bio engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Dmitrii M NikolaevInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Ekaterina M MetelkinaInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Nikita A DomskiiInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Ilya R OsadchyInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Andrey S MereshchenkoInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.ORCID https://orcid.org/0000-0001-9390-1446
Stanislav A BondarevDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya emb, St. Petersburg 199034, Russia.ORCID https://orcid.org/0000-0003-3137-944X
Galina A ZhouravlevaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya emb, St. Petersburg 199034, Russia.
Andrey A ShtyrovInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Maxim S PanovInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Mikhail N RyazantsevInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.ORCID https://orcid.org/0000-0003-3413-1706

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A semirational engineering of novel bright variants of archaerhodopsin-based genetically encoded voltage indicators (GEVIs) is performed. The corresponding focused protein sequence library was generated using data derived from prior directed evolution experiments, computational modeling, evolutionary information, and a physics-based theoretical framework. The proposed variants were synthesized in

Indexed as

fluorescent rhodopsinsgenetically encoded voltage indicatorsGEVImembrane potentialoptogeneticsprotein engineeringsemi-rational protein engineering

Identifiers

PMID41158609
PMCPMC12557449

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.