Evidence map›Paper›PMID 41157626›Full record

ArticleViruses2025

Standardization of Quantitative Plaque-Based Viral Assays for Orthoflavivirus Cacipacoré.

Marielena Vogel Saivish, Natalia I O da Silva, Madeline R Steck, Rafael E Marques, Mauricio L Nogueira, Shannan L Rossi, Nikos Vasilakis

Abstract read
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Marielena Vogel SaivishLaboratórios de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto 15090-000, SP, Brazil.ORCID 0000-0002-7627-621X
Natalia I O da SilvaDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA.
Madeline R SteckDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA.ORCID 0009-0004-4160-0325
Rafael E MarquesLaboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas 13083-100, SP, Brazil.ORCID 0000-0002-6949-0947
Mauricio L NogueiraLaboratórios de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto 15090-000, SP, Brazil.ORCID 0000-0003-1102-2419
Shannan L RossiDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA.ORCID 0000-0003-2450-2374
Nikos VasilakisDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA.ORCID 0000-0002-0708-3289

Funding

Project 5: mAb Development Flaviviruses and AlphavirusesU19AI181960 · NIAID · WASHINGTON UNIVERSITY · PI DIAMOND, MICHAEL S, KUHN, RICHARD J. · 2024 to 2024
$46.7M
Medicinal Chemistry Optimization of Anti-Alphaviral Leads and Elucidation of Target and Off Target EngagementU19AI142762 · NIAID · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI CHUNG, DONGHOON · 2019 to 2023
$22.0M
Coordinating Research on Emerging Arboviral Threats Encompassing the Neotropics II (CREATE-NEO II)U01AI151807 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI Kathryn Alyce Hanley, MAURICIO Lacerda NOGUEIRA · 2020 to 2026
$9.8M
World Reference Center for Emerging Viruses and Arboviruses (WRCEVA)R24AI120942 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI WEAVER, SCOTT C · 2016 to 2024
$8.5M
EMERGING AND TROPICAL INFECTIOUS DISEASEST32AI007526 · NIAID · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI SOONG, LYNN · 1997 to 2025
$3.1M
Centers for Research in Emerging Infectious Diseases (CREID), "The Coordinating Research on Emerging Arboviral Threats Encompassing the Neotropics (CREATE-NEO) U01AI151807Fundação de Amparo à Pesquisa do Estado de São Paulo 2013/21719-3Fundação de Amparo à Pesquisa do Estado de São Paulo 2020/12875-5Fundação de Amparo à Pesquisa do Estado de São Paulo 2022/03645-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/09590-7INCT Viral Genomic Surveillance and One Health grant 405786/2022-0National Institutes of Health (NIH) U19AI142762NIAID NIH HHS R24 AI120942NIAID NIH HHS T32 AI007526NIAID NIH HHS U01 AI151807NIAID NIH HHS U19 AI142762NIAID NIH HHS U19 AI181960NIH HHS 1T32AI007526-25NIH HHS 1U19AI181960-25
6 · The paper itself

Abstract

Cacipacoré virus (CPCV) is an understudied orthoflavivirus with significant gaps in research that hinders our understanding of its ecology, host range, and potential public health impact. A notable barrier to filling this gap is the absence of standardized methodologies for viral quantification, such as plaque-forming and focus-forming assays. This short communication outlines the development of optimized protocols for CPCV titration in two cell lines, Vero CCL-81 and BHK CCL-10, using several overlay and time point conditions. These methodologies address the need for robust quantitative tools to advance research on CPCV and its implications for human and animal health, fostering progress in the surveillance and understanding of this underexplored arbovirus.

Indexed as

FlaviviridaeViral LoadViral Plaque AssayAnimalsCell LineChlorocebus aethiopsCricetinaeHumansVero CellsCacipacoré virusemerging infectious diseasesfamily Flaviviridaegenus Orthoflavivirus

Identifiers

PMID41157626
PMCPMC12568257

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.