Evidence map›Paper›PMID 41157182›Full record

ArticleLife (Basel, Switzerland)2025

Short-Term In Vitro Culture of Human Ovarian Tissue: A Comparative Study of Serum Supplementation for Primordial Follicle Survival.

Serena Marcozzi, Rossella Vicenti, Gina La Sala, Harpreet Kaur Lamsira, Catello Scarica, Nicole Bertani, Massimo De Felici, Raffaella Fabbri, Francesca Gioia Klinger

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Life (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Serena MarcozziSection of Histology and Embryology, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.ORCID 0000-0001-5873-5753
Rossella VicentiDivision of Gynaecology and Human Reproduction Physiopathology, IRCCS Azienda Ospedaliera-Universitaria di Bologna, 40100 Bologna, Italy.
Gina La SalaInstitute of Biochemistry and Cell Biology, Italian National Research Council (CNR), 00015 Monterotondo Scalo, Italy.ORCID 0000-0002-1907-5948
Harpreet Kaur LamsiraSection of Histology and Embryology, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
Catello ScaricaNew Fertility Group Centre for Reproductive Medicine, European Hospital, 00100 Rome, Italy.ORCID 0000-0001-7630-6880
Nicole BertaniSection of Histology and Embryology, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
Massimo De FeliciSection of Histology and Embryology, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.ORCID 0000-0001-8479-8264
Raffaella FabbriDivision of Gynaecology and Human Reproduction Physiopathology, IRCCS Azienda Ospedaliera-Universitaria di Bologna, 40100 Bologna, Italy.
Francesca Gioia KlingerSaint Camillus International University of Health Sciences, 00131 Rome, Italy.ORCID 0000-0001-6744-0850

Funding

Italian Ministry of University and Research PRIN grant 2022 FFert-REPAIR N. 2022A24YYY
6 · The paper itself

Abstract

Optimizing in vitro culture conditions for cryopreserved-thawed human ovarian cortical fragments (OCFs) represents a critical step in fertility preservation strategies. OCFs predominantly contain primordial follicles (PMFs), whose survival and integrity are essential for ex vivo folliculogenesis. This study aimed to evaluate the impact of different culture media supplementations on PMF survival and tissue morphology by comparing alpha-Minimum Essential Medium (αMEM) supplemented with Human Serum Albumin (HSA), Human Serum (HS), or Serum Substitute Supplement (SSS). Twenty-nine OCFs were cultured for three days, and follicular density and were morphology assessed. Generalized linear mixed model analysis showed that PMF density was significantly higher in OCFs cultured in medium supplemented with SSS (213 PMFs/mm

Indexed as

fertility preservationin vitro culturemedium supplementsovarian cortical tissueovarian follicles

Identifiers

PMID41157182
PMCPMC12565730

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.