ArticlePathogens (Basel, Switzerland)2025
Specificity of Primers and Probes for Molecular Diagnosis of
Giovanna Zandonadi Haber, Leila Dias da Costa, Erick Bruno Monteiro Costa, Railton Farias Araújo, Tainá Negreiros de Souza, Luciana do Rêgo Lima Queiroz, Bruno Tardelli Nunes Diniz, Edivaldo Costa Sousa Junior, Lívia Carício Martis, Patrícia Karla Santos Ramos and 1 more
Abstract read
In one paragraphArticle in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
11 authors.
Giovanna Zandonadi HaberParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.ORCID 0009-0001-4061-9145 Leila Dias da CostaParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.
Erick Bruno Monteiro CostaParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.
Railton Farias AraújoParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.
Tainá Negreiros de SouzaParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.ORCID 0009-0009-7370-5486 Luciana do Rêgo Lima QueirozParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.ORCID 0000-0002-3240-2835 Bruno Tardelli Nunes DinizArbovirus Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.ORCID 0000-0002-0793-9665 Edivaldo Costa Sousa JuniorParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.
Lívia Carício MartisArbovirus Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.ORCID 0000-0002-9400-2036 Patrícia Karla Santos RamosParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.
Fernando Tobias SilveiraParasitology Department, Evandro Chagas Institute (Health and Environment Surveillance Secretariat, Ministry of Health), Ananindeua 67030-000, Pará, Brazil.ORCID 0000-0002-0412-6060 Funding
No grant is acknowledged in the PubMed record.
6 · The paper itselfAbstract
Molecular tools, especially real-time polymerase chain reaction (qPCR), are relevant tools for laboratory diagnosis due to their sensitivity, specificity, rapid results, and ability to quantify parasite load. This study evaluated the specificity of the LEISH-1/LEISH-2 primer pair with the TaqMan MGB probe in serum samples previously classified by indirect Enzyme-Linked Immunosorbent Assay (ELISA) (30 positive dogs, 30 negative dogs; 9 positive wild animals and 16 negative wild animals) using in silico analyses (Primer-BLAST, Multiple Alignment using Fast Fourier Transform-MAFFT
Indexed as
DNA PrimersDog DiseasesLeishmaniaLeishmaniasisLeishmaniasis, VisceralMolecular Diagnostic TechniquesAnimalsAnimals, WildDogsReal-Time Polymerase Chain ReactionSensitivity and SpecificityDNA Primersdogsmolecular diagnosisprimersqPCRTaqMan probevisceral leishmaniasiswild animals
Identifiers
PMID41156675
PMCPMC12566895
What OpenQuestion holds
Textmetadata
LicenceCC BY
Read underepoch 390