Evidence map›Paper›PMID 41156661›Full record

ArticlePathogens (Basel, Switzerland)2025

Comparative Analysis of Concentration and Quantification Methods for Antibiotic Resistance Genes and Their Phage-Mediated Dissemination in Treated Wastewater and Biosolids.

Irene Falcó, Ana Allende, Francesca Cutrupi, Rosa Aznar, Gloria Sánchez, Pilar Truchado

Abstract readComparative Study
In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Irene FalcóDepartment of Microbiology and Ecology, University of Valencia, 46100 Valencia, Spain.ORCID 0000-0002-4036-3274
Ana AllendeResearch Group on Microbiology and Quality of Fruits and Vegetables (MxQ), Department of Food Science and Technology, CEBAS-CSIC, Campus Universitario de Espinardo, 25, 30100 Murcia, Spain.
Francesca CutrupiCIBIO-Department of Cellular, Computational and Integrative Biology, University of Trento, 38123 Trento, Italy.ORCID 0000-0002-3097-5592
Rosa AznarDepartment of Microbiology and Ecology, University of Valencia, 46100 Valencia, Spain.ORCID 0000-0002-8761-8887
Gloria SánchezEnvironmental Virology and Food Safety Lab (VISAFELab), Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, 46980 Valencia, Spain.ORCID 0000-0001-7022-661X
Pilar TruchadoResearch Group on Microbiology and Quality of Fruits and Vegetables (MxQ), Department of Food Science and Technology, CEBAS-CSIC, Campus Universitario de Espinardo, 25, 30100 Murcia, Spain.

Funding

project MCEC IMAGE (PID 2020 116789 RB C 42 AEI/FEDER, UE), PTI Salud Global CSIC, the Spanish Ministry of Science and Innovation (Project TED2021-131427B-C21 and TED2021-131427B-C22), as well as the a Fundación Séneca - Agencia de Ciencia y Tecnología de project MCEC IMAGE (PID 2020 116789 RB C 42 AEI/FEDER, UE), PTI Salud Global CSIC, the Spanish Ministry of Science and Innovation (Project TED2021-131427B-C21 and TED2021-131427B-C22), as well as the a Fundación Séneca - Agencia de Ciencia y Tecnología de
6 · The paper itself

Abstract

Antimicrobial resistance poses a growing threat to public health, and integrated surveillance strategies across environmental compartments such as treated wastewater and biosolids can substantially improve monitoring efforts. A key challenge is the diversity of available protocols, which complicates comparability for the concentration and detection of antibiotic resistance genes (ARGs), particularly in complex matrices. In this study, we compared two commonly used concentration methods-filtration-centrifugation (FC) and aluminum-based precipitation (AP)-and two detection techniques, quantitative PCR (qPCR) and droplet digital PCR (ddPCR), for the quantification of four clinically relevant ARGs:

Indexed as

BacteriophagesDrug Resistance, BacterialDrug Resistance, MicrobialGenes, BacterialSewageWastewaterReal-Time Polymerase Chain ReactionSewageWastewaterantimicrobial resistance genesbacteriophagesbiosolidsdigital PCRwastewater

Identifiers

PMID41156661
PMCPMC12566704

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.