Evidence map›Paper›PMID 41156591›Full record

ArticlePathogens (Basel, Switzerland)2025

Rapid Detection of Duck Enteritis Virus with MIRA, MIRA-qPCR, and MIRA-LFD Assays.

Yin Dai, Xiaomiao Hu, Yueyi Zhong, Liyuan Chen, Jieru Wang, Dongdong Yin, Lei Yin, Xuehuai Shen, Xiaocheng Pan, Xuelan Liu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yin DaiAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.
Xiaomiao HuAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.
Yueyi ZhongCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China.
Liyuan ChenAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.
Jieru WangAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.ORCID 0000-0002-9332-6514
Dongdong YinAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.
Lei YinAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.
Xuehuai ShenAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.ORCID 0000-0002-7558-8123
Xiaocheng PanAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.
Xuelan LiuCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China.
Ruihong ZhaoAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China.

Funding

the Major Science and Technology Special Project in Anhui Province 202003a06020012
6 · The paper itself

Abstract

Duck viral enteritis (DVE) is an acute and highly contagious disease that affects waterfowl such as ducks, geese and swans. Duck enteritis virus (DEV) is the pathogen, causing huge economic losses to waterfowl farming in recent years. Establishing a rapid, simple, and visual detection should facilitate the early identification of DEV. After the amplification primers and reaction conditions were optimized, three multienzyme isothermal rapid amplification (MIRA) methods-basic MIRA, MIRA-quantitative PCR (MIRA-qPCR) and MIRA-lateral flow dipstick (MIRA-LFD)-were established to detect DEV. Specificity analyses showed that the three MIRA methods specifically detected DEV, with no cross-reaction with fowl adenovirus serotype 4, novel goose astrovirus, Muscovy duck reovirus, avian influenza virus subtype H9, or duck circovirus. The basic MIRA reaction was completed in 30 min at 35 °C, requiring only a pair of primers. Detection with MIRA-qPCR or MIRA-LFD was completed within 20 min, and the limits of detection were 1 × 10

Indexed as

EnteritisMardivirusMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPoultry DiseasesSiadenovirusAnimalsDucksReal-Time Polymerase Chain ReactionSensitivity and Specificityduck enteritis virusmultienzyme isothermal rapid amplificationsensitivityspecificityvisible detection

Identifiers

PMID41156591
PMCPMC12566609

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.