ArticleBiomolecules2025
Engineering a High-Fidelity MAD7 Variant with Enhanced Specificity for Precision Genome Editing via CcdB-Based Bacterial Screening.
Haonan Zhang, Ying Yang, Tianxiang Yang, Peiyao Cao, Cheng Yu, Liya Liang, Rongming Liu, Zhiying Chen
Abstract read
In one paragraphArticle in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
8 authors.
Haonan ZhangCentral Hospital of Dalian University of Technology, Xinan Road No. 826, Dalian 116024, China.
Ying YangCentral Hospital of Dalian University of Technology, Xinan Road No. 826, Dalian 116024, China.
Tianxiang YangMOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Linggong Road No. 2, Dalian 116024, China.
Peiyao CaoMOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Linggong Road No. 2, Dalian 116024, China.
Cheng YuMOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Linggong Road No. 2, Dalian 116024, China.
Liya LiangMOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Linggong Road No. 2, Dalian 116024, China.ORCID 0009-0003-7346-1991 Rongming LiuMOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Linggong Road No. 2, Dalian 116024, China.ORCID 0009-0001-2681-8164 Zhiying ChenCentral Hospital of Dalian University of Technology, Xinan Road No. 826, Dalian 116024, China.
Funding
Fundamental Research Funds for the Central Universities DUT24YG131Fundamental Research Funds for the Central Universities DUT25LAB105Fundamental Research Funds for the Central Universities DUT25RC(3)019National Key R&D Program of China 2023YFC3402300National Natural Science Foundation of China 22208044National Natural Science Foundation of China 22278058Natural Science Foundation of Liaoning Province 2024-MSBA-09Natural Science Foundation of Liaoning Province 2025JH2/101330156Science and Technology Innovation Foundation of Dalian 2023JJ12SN030"Xingliao Talent Plan"project XLYC2203075
6 · The paper itselfAbstract
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) nucleases enable precise genome editing, but off-target cleavage remains a critical challenge. Here, we report the development of MAD7_HF, a high-fidelity variant of the MAD7 nuclease engineered through a bacterial screening system leveraging the DNA gyrase-targeting toxic gene
Indexed as
Bacterial ProteinsEndonucleasesEscherichia coliGene EditingCRISPR-Cas SystemsMutationRNA, Guide, CRISPR-Cas SystemsBacterial ProteinsEndonucleasesRNA, Guide, CRISPR-Cas Systemsbacterial screening systemhigh specificityMAD7_HFoff-target
Identifiers
PMID41154642
PMCPMC12563919
What OpenQuestion holds
Textmetadata
LicenceCC BY
Read underepoch 390