Evidence map›Paper›PMID 41154633›Full record

ArticleBiomolecules2025

Thermodynamic Determinants in Antibody-Free Nucleic Acid Lateral Flow Assays (AF-NALFA): Lessons from Molecular Detection of

Leonardo Lopes-Luz, Paula Correa Neddermeyer, Gabryele Cardoso Sampaio, Luana Michele Alves, Matheus Bernardes Torres Fogaça, Djairo Pastor Saavedra, Mariane Martins de Araújo Stefani, Samira Bührer-Sékula

Abstract read
In one paragraph

Article in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Leonardo Lopes-LuzInstituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania 74605-050, Brazil.ORCID 0000-0003-0258-9026
Paula Correa NeddermeyerInstituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania 74605-050, Brazil.ORCID 0009-0007-7657-6224
Gabryele Cardoso SampaioInstituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania 74605-050, Brazil.
Luana Michele AlvesInstituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania 74605-050, Brazil.
Matheus Bernardes Torres FogaçaInstituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania 74605-050, Brazil.ORCID 0000-0002-8465-6124
Djairo Pastor SaavedraInstituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania 74605-050, Brazil.ORCID 0000-0002-2664-3800
Mariane Martins de Araújo StefaniInstituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania 74605-050, Brazil.
Samira Bührer-SékulaInstituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania 74605-050, Brazil.ORCID 0000-0002-5984-7770

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior 88881.082898/2024-01Coordenação de Aperfeicoamento de Pessoal de Nível Superior 88887.159650/2025-00Coordenação de Aperfeicoamento de Pessoal de Nível Superior 8888.7940507/2024-00Coordenação de Aperfeicoamento de Pessoal de Nível Superior 88887.940841/2024-00Coordenação de Aperfeicoamento de Pessoal de Nível Superior 88887.974403/2024-00Financiadora de Estudos e Projetos 01.23.0028.00Fundação de Apoio a Pesquisa do Estado de Goiás 202510267001083National Council for Scientific and Technological Development 380387/2025-5National Council for Scientific and Technological Development 443185/2024-7
6 · The paper itself

Abstract

Antibody-free nucleic acid lateral flow assays (AF-NALFA) are an established approach for rapid detection of amplified pathogens DNA but can yield inconsistent signals across targets. Since AF-NALFA depends on dual hybridization of probes to single-stranded amplicons (ssDNA), site-specific thermodynamic (Gibbs free energy-ΔG) at probe-binding regions may be crucial for performance. This study investigated how

Indexed as

LeishmaniaListeria monocytogenesMycobacterium lepraeDNA, Single-StrandedNucleic Acid HybridizationThermodynamicsDNA, Single-StrandedDNA hybridizationGibbs free energylateral flow testsmismatchespathogen detectionpoint-of-care DNA Detectionsingle-nucleotide polymorphism

Identifiers

PMID41154633
PMCPMC12562187

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.