ArticleGenes2025
Gene Mapping and Molecular Marker Development for Controlling Purple-Leaf Trait in Pakchoi (
Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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13 authors.
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Abstract
backgroundspurple pakchoi (
methodsIn this study, we used the inbred purple-leaf line 'PQC' and green-leaf line 'HYYTC' as parents to construct a six-generation genetic segregation population. We analyzed the inheritance pattern of the purple-leaf trait and combined Bulked Segregant Analysis Sequencing (BSA-Seq) with penta-primer amplification refractory mutation system (PARMS) to map the causal gene.
resultsthe main findings are as follows: the purple-leaf trait is controlled by a single dominant gene. Using BSA-Seq and PARMS, the genes were mapped to a 470 kb region (31.18-31.65 Mb) on chromosome A03. Within this interval, 29 candidate genes were identified,
conclusionsthe candidate gene controlling purple-leaf trait was finally located to A03 chromosome 31.18-31.65 Mb. The SNP31304070 marker and trait were co-separated, This marker could be applied to molecular-assisted breeding in purple pakchoi.
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