ArticleGenes2025
Genome-Wide Identification and Expression Analysis of the bHLH Transcription Factor Family in
Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
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Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
BACKGROUND/
objectivesThe basic helix-loop-helix (bHLH) transcription factor family regulates plant development, metabolism, and stress responses. Yet, its genome-wide composition remains unexplored in
methodsPacBio HiFi full-length transcriptome sequencing was combined with Illumina RNA-seq for accurate structural annotation and expression quantification. Candidate bHLHs were identified by iTAK and HMMER-Pfam, and their physicochemical properties, secondary structures, motifs, and phylogenetic positions were examined. Expression patterns were analyzed across four floral stages (bud, initial bloom, full bloom, and late bloom).
resultsA total of 113 high-confidence bHLH genes were identified, with ~90% successfully annotated. The proteins displayed variation in molecular weight, isoelectric point, structural features, and motif composition. Phylogenetic analysis placed them into 13 clades consistent with
conclusionsThis work provides the first genome-wide characterization of bHLHs in LBVR. The integrated sequencing approach generated a robust catalogue and developmental expression map, offering candidates for functional studies and resources for breeding in lilies.
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