Evidence map›Paper›PMID 41152411›Full record

ArticleCommunications biology2025

Exposure of mice to environmentally relevant per- and polyfluoroalkyl substances (PFAS) alters the sperm epigenome.

Leah Gillespie, Jacinta H Martin, Amanda L Anderson, Ilana R Bernstein, Simone J Stanger, Natalie A Trigg, John E Schjenken, Anne-Louise Gannon, Shanu Parameswaran, Shannon P Smyth and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Endocrine Disruption and Male Fertility.Current environmental health reports · 2026
    Review
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Leah Gillespie *School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Jacinta H Martin *School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia. jacinta.martin@newcastle.edu.au.ORCID http://orcid.org/0000-0002-0876-5150
Amanda L AndersonSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Ilana R BernsteinSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Simone J StangerSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Natalie A TriggSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
John E SchjenkenSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.ORCID http://orcid.org/0000-0001-6293-6160
Anne-Louise GannonSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Shanu ParameswaranSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Shannon P SmythSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Colin C ConineDivision of Neonatology, Children's Hospital of Philadelphia, Philadelphia, PA, USA.ORCID http://orcid.org/0000-0001-7915-0463
Reena DesaiANZAC Research Institute, University of Sydney, Sydney, NSW, Australia.
David J HandelsmanANZAC Research Institute, University of Sydney, Sydney, NSW, Australia.ORCID http://orcid.org/0000-0002-4200-7476
Geoffry N De IuliisSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.ORCID http://orcid.org/0000-0002-6978-6123
Andrew L EamensSchool of Health, University of the Sunshine Coast, Maroochydore, QLD, Australia.ORCID http://orcid.org/0000-0002-6327-3618
Matthew D DunCancer Signalling Research Group, School of Biomedical Sciences and Pharmacy, College of Health, Medicine and Wellbeing, University of Newcastle, Callaghan, NSW, Australia.ORCID http://orcid.org/0000-0002-9063-5370
Brett D TurnerCentre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW, Australia.
Shaun D RomanNSW Health Pathology, Newcastle, NSW, Australia.ORCID http://orcid.org/0000-0002-7758-7757
Mark P GreenSchool of BioSciences, Faculty of Science, University of Melbourne, Melbourne, VIC, Australia.
Brett NixonSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.ORCID http://orcid.org/0000-0003-2745-8188

Funding

Translational Research Support CoreP30ES013508 · NIEHS · UNIVERSITY OF PENNSYLVANIA · PI A. Clementina Mesaros · 2006 to 2026
$35.3M
Department of Health | National Health and Medical Research Council (NHMRC) APP1154837Department of Health | National Health and Medical Research Council (NHMRC) APP1173892Department of Health | National Health and Medical Research Council (NHMRC) APP1189415NIEHS NIH HHS P30 ES013508
6 · The paper itself

Abstract

Per- and polyfluoroalkyl substances (PFAS) are a large group of persistent synthetic chemicals and ubiquitous environmental contaminants. Mounting evidence demonstrates that PFAS can bioaccumulate and induce adverse health outcomes, including compromising male reproduction. Despite this, the mechanisms by which PFAS elicits these effects remain unclear. Here, we investigate how an environmentally relevant PFAS cocktail impacts the reproductive function of male Swiss CD1 mice. Following twelve weeks of continuous exposure, we collected blood samples for hormone and PFAS quantification and processed reproductive tissues and spermatozoa for histological and functional assessment. PFAS exposure significantly reduced the rate of daily sperm production, likely due to decreased circulating testosterone and dihydrotestosterone. Further, PFAS-exposed spermatozoa displayed marked alterations to their small non-coding RNA profile, which were linked to dysregulation of early-embryonic gene expression. Notably, these changes occured without significant alteration in sperm viability, motility, or the ability to undergo capacitation or support embryonic development. These findings provide new mechanistic insight into how PFAS exposure impacts male reproductive health.

Indexed as

Environmental ExposureEnvironmental PollutantsEpigenomeFluorocarbonsSpermatozoaAnimalsMaleMiceSperm MotilityEnvironmental PollutantsFluorocarbons

Identifiers

PMID41152411
PMCPMC12568938

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.