Evidence map›Paper›PMID 41150736›Full record

ArticleCurrent issues in molecular biology2025

The Time of Blood Collection Does Not Alter the Composition of Leucocyte-Poor Platelet-Rich Plasma: A Quantitative Analysis of Platelets and Key Regenerative Proteins.

Hadrian Platzer, Alena Bork, Malte Wellbrock, Axel Horsch, Ghazal Pourbozorg, Simone Gantz, Reza Sorbi, Sébastien Hagmann, Yannic Bangert, Babak Moradi

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Article in Current issues in molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hadrian PlatzerDepartment of Orthopedics and Trauma Surgery, University Medical Center Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105 Kiel, Germany.
Alena BorkDepartment of Orthopedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, Germany.
Malte WellbrockOrthopedic Research Center, Kiel University, Michaelisstr. 5, 24105 Kiel, Germany.ORCID 0009-0004-2481-7151
Axel HorschDepartment of Orthopedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, Germany.ORCID 0000-0002-6487-030X
Ghazal PourbozorgOrthopedic Research Center, Kiel University, Michaelisstr. 5, 24105 Kiel, Germany.
Simone GantzOrthopedic Research Center, Kiel University, Michaelisstr. 5, 24105 Kiel, Germany.ORCID 0009-0006-2075-9550
Reza SorbiDepartment of Orthopedics and Trauma Surgery, University Medical Center Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105 Kiel, Germany.ORCID 0009-0005-8877-1102
Sébastien HagmannDepartment of Orthopedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, Germany.
Yannic BangertDepartment of Orthopedics, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, Germany.ORCID 0000-0001-8392-7829
Babak MoradiDepartment of Orthopedics and Trauma Surgery, University Medical Center Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105 Kiel, Germany.ORCID 0000-0003-1714-2171

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Platelet-rich plasma (PRP) is increasingly used in orthopedics, with its regenerative potential attributed to platelet-derived cytokines and growth factors. However, variability in PRP composition hampers standardization and reproducibility, contributing to inconsistent outcomes. Since platelet counts in whole blood follow circadian rhythms, we investigated whether blood collection time affects PRP composition. Venous blood was collected from 25 healthy individuals at 8:00 a.m., 12:00 p.m., and 4:00 p.m. Whole blood was analyzed, and leukocyte-poor PRP (LP-PRP) was prepared for quantification of erythrocytes, leukocytes, and platelets. Platelets were lysed by freeze-thaw cycles, and protein levels of PDGF-BB, IGF1, HGF, IL6, and IL10 were measured via ELISA. Whole blood exhibited diurnal variation in platelet counts. In contrast, LP-PRP was consistently depleted of leukocytes and erythrocytes and showed stable platelet enrichment (PRP/whole blood ratio 2.1 ± 0.3). Protein concentrations in LP-PRP did not differ across collection times. Despite marked interindividual variability, no time-dependent differences were observed in platelet or protein composition. These results demonstrate that LP-PRP is temporally stable, independent of blood sampling time. This robustness supports flexible clinical use and contributes to efforts toward PRP standardization in therapeutic practice.

Indexed as

blood collection timecytokinesgrowth factorsleucocyte-poor platelet-rich plasmaPRPregenerative proteins

Identifiers

PMID41150736
PMCPMC12564387

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