In Vitro Effect of Elevated Ammonia and Urea Levels on Post-Thawed Bull Semen Sperm Characteristics.

Amine Abdelli, Mohamed Besbaci, Ziyad Al-Kass, Mokrane Iguer-Ouada, Jane M Morrell

Abstract read

In one paragraph

Article in Veterinary sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Amine AbdelliDepartment of Agricultural Sciences, Laboratory of Management and Valorization of Natural Resources and Quality Assurance (LGVRNAQ), University of Bouira, Bouira 10000, Algeria.ORCID 0000-0001-9977-1232

Mohamed BesbaciLaboratory of Biotechnology in Animal Reproduction, Institute of Veterinary Medicine, University of Blida1, Blida 09000, Algeria.ORCID 0000-0002-7934-6359

Ziyad Al-KassDepartment of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul 41003, Iraq.ORCID 0000-0002-2933-2258

Mokrane Iguer-OuadaFaculty of Nature and Life Sciences, Associated Laboratory in Marine Ecosystems and Aquaculture, University of Bejaia, Bejaia 06000, Algeria.

Jane M MorrellDivision of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden.ORCID 0000-0002-5245-7331

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Elevated ammonia and urea, common byproducts of nitrogen metabolism, are increasingly found in dairy cows and may negatively impact reproductive function. However, their effects on bull sperm remain unclear. This study aimed to assess the impact of ammonia and urea at physiologically relevant concentrations on key sperm characteristics. Thawed bull semen was incubated under five treatment conditions: control (no added ammonia or urea), low urea (LU), high urea (HU), low ammonia (LA), and high ammonia (HA). Sperm motility and kinematics were assessed using Computer-Assisted Semen Analysis (CASA), and sperm viability, DNA integrity, mitochondrial membrane potential (MMP), and oxidative stress markers were evaluated using flow cytometry. HA significantly reduced MMP (

Indexed as

ammoniabovineco-incubationsemen characteristicsurea

Identifiers

PMID41150137

DOI10.3390/vetsci12100997

PMCPMC12568276

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.