ArticleeLife2025
Deep3DSIM: Super-resolution imaging of thick tissue using 3D structured illumination with adaptive optics.
Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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Who cites it
3 citing papers in PubMed.
- SIMworks-an integrated software suite for quality-controlled augmented quantitative structured illumination microscopy.Nature protocols · 2026Review
- Implementation of an adaptive-optics assisted isoSTED nanoscope.Nature protocols · 2026Review
- Phase-based computational adaptive optics enables artifact-free super-resolution microscopy.Communications engineering · 2026Article
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
Three-dimensional structured illumination microscopy (3D-SIM) doubles the resolution of fluorescence imaging in all directions and enables optical sectioning with increased image contrast. However, 3D-SIM has not been widely applied to imaging deep in thick tissues due to its sensitivity to sample-induced aberrations, making the method difficult to apply beyond 10 µm in depth. Furthermore, 3D-SIM has not been available in an upright configuration, limiting its use for live imaging while manipulating the specimen, for example, with electrophysiology. Here, we have overcome these barriers by developing a novel upright 3D-SIM system (termed Deep3DSIM) that incorporates adaptive optics for aberration correction and remote focusing, reducing artefacts, improving contrast, restoring resolution, and eliminating the need to move the specimen or the objective lens in volume imaging. These advantages are equally applicable to inverted 3D-SIM systems. We demonstrate high-quality 3D-SIM imaging in various samples, including imaging more than 130 µm into the
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