Evidence map›Paper›PMID 41148645›Full record

ArticleInfectious disease reports2025

Development of a PCR Assay for the Detection of

William N Bélanger, Martine Bastien, Eve Bérubé, Martin Gagnon, Yesmine G Sahnoun, Valérie Dancause, Karel Boissinot, Cindy Lalancette, Christian Riel-Roberge, Marieve Jacob-Wagner and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infectious disease reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

William N BélangerDépartement de Biochimie, Microbiologie et Bio-Informatique, Faculté Des Sciences et de Génie, Université Laval, Quebec City, QC G1V 0A6, Canada.
Martine BastienAxe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Quebec City, QC G1V 4G2, Canada.
Eve BérubéAxe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Quebec City, QC G1V 4G2, Canada.
Martin GagnonAxe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Quebec City, QC G1V 4G2, Canada.
Yesmine G SahnounAxe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Quebec City, QC G1V 4G2, Canada.
Valérie DancauseProgramme de Prévention et de Contrôle des Infections, CHU de Québec-Université Laval, Quebec City, QC G1R 2J6, Canada.
Karel BoissinotDépartement de Microbiologie et D'infectiologie, CHU de Québec-Université Laval, Quebec City, QC G1S 4L8, Canada.
Cindy LalancetteLaboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, QC H9X 3R5, Canada.
Christian Riel-RobergeDirection de Santé Publique, Centre Intégré Universitaire de Santé et de Services Sociaux (CIUSSS) de la Capitale-Nationale, Quebec City, QC G1E 7G9, Canada.
Marieve Jacob-WagnerDépartement de Microbiologie et D'infectiologie, CHU de Québec-Université Laval, Quebec City, QC G1S 4L8, Canada.
Sylvie TrottierAxe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Quebec City, QC G1V 4G2, Canada.ORCID 0000-0002-3986-5146
Damien Biot-PelletierCentre D'expertise en Analyse Environnementale du Québec (CEAEQ), Gouvernement du Québec, Quebec City, QC G1P 3W8, Canada.ORCID 0000-0002-3278-1345
Annie RuestDépartement de Microbiologie et D'infectiologie, CHU de Québec-Université Laval, Quebec City, QC G1S 4L8, Canada.
Isabelle TétreaultDépartement de Microbiologie et D'infectiologie, CHU de Québec-Université Laval, Quebec City, QC G1S 4L8, Canada.
Mathieu ThériaultAxe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Quebec City, QC G1V 4G2, Canada.ORCID 0000-0002-7293-7623
Sandra IsabelAxe Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Quebec City, QC G1V 4G2, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

objectives

methodsWe targeted the 23S-5S intergenic spacer region, which can differentiate

resultsThe PCR assay demonstrated 100% analytical specificity (no cross-reactivity with non-targeted species), 100% inclusivity (detection of all

conclusionsThis PCR assay enables accurate detection of

Indexed as

23S–5S intergenic spacerenvironmental detectionLegionella micdadeiPCR assay

Identifiers

PMID41148645
PMCPMC12564405

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.