Effective Anti-Cancer Activity of Red Rice Bran Extract on Cervical Cancer and Breast Cancer Cells.

objectiveMany researchers have shown that red rice bran is a rich source of antioxidants and has potential anticancer properties. This research aims to evaluate the cytotoxicity and inhibitory effects of Red Rice Bran Extract (RRBE) on the growth and migration of human cervical and breast cancer cells.

methodsHeLa and MCF-7 cancer cells were treated with various concentrations of RRBE for 24 and 48 h. Cytotoxicity was assessed using the MTT assay. Then, the half-inhibitory concentration (IC50) value was determined. The anti-proliferative effects were evaluated using the MTS assay, and the anti-cell migration activity was assessed using the wound healing assay.

resultIt was found that the RRBE exhibited cytotoxicity to both HeLa and MCF-7 cancer cells. As the concentration level increased (100 - 1000 µg/ml), the survival rate of the cells significantly decreased, especially at 48 h, corresponding to the IC50 values of 818.67 µg/ml and >1000 µg/ml for HeLa and MCF-7, respectively. Then, concentrations of RRBE with lower cytotoxic effects were selected for further experiments, which revealed that treatment with RRBE at concentrations of 100, 250, 500, and 1000 µg/ml significantly suppressed the proliferation and reduced the migration of those cancer cells.

conclusionThese results demonstrate that RRBE has potent anti-cancer activity in HeLa and MCF-7 cancer cells, making it a suitable candidate for in vivo studies and development as an anti-cancer agent.