Evidence map›Paper›PMID 41148460›Full record

ArticleActa parasitologica2025

First Molecular Characterization of Cryptosporidium and Giardia from Pigs in Bangladesh.

Tania Sultana, Anas Bin Harun, Abdullah Al Bayazid, Md Farhan Hasan, Ainun Nahar, Jinnat Rehena, Sourov Sutradhar, Joynti Saha, S H M Faruk Siddiki, M Nazmul Hoque and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Acta parasitologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Tania SultanaDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Anas Bin HarunDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Abdullah Al BayazidDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Md Farhan HasanDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Ainun NaharDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Jinnat RehenaDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Sourov SutradharZoonosis Research Laboratory, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Joynti SahaDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
S H M Faruk SiddikiDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
M Nazmul HoqueDepartment of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Amam Zonaed SiddikiDepartment of Pathology and Parasitology, Chattogram Veterinary and Animal Sciences University, Chattogram, 4202, Bangladesh.
Junqiang LiCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.
Md Robiul KarimDepartment of Medicine, Gazipur Agricultural University, Gazipur, 1706, Bangladesh. vet_robiul@gau.edu.bd.

Funding

Research Management Wing (RMW) of Gazipur Agricultural University (GAU), Bangladesh 026
6 · The paper itself

Abstract

purposeCryptosporidium and Giardia are significant causes of gastrointestinal diseases in humans and animals worldwide, especially in pigs. This study investigated the molecular occurrence, genetic diversity, and zoonotic potential of Cryptosporidium spp. and Giardia duodenalis in domestic pigs in the Chattogram Hill Tracts (CHT), Bangladesh.

methodsA total of 408 fecal samples were collected from Rangamati, Khagrachari, and Bandarban districts and examined using nested PCR, targeting the SSU rRNA gene for Cryptosporidium and the β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes for G. duodenalis. Nucleotide sequencing and phylogenetic analysis were conducted for molecular characterization.

resultsThe prevalence of Cryptosporidium was 3.43%, and G. duodenalis was 23.52%. The prevalence varied by several factors, although the variations were not significant for Cryptosporidium. Age, anthelmintic administration, and diarrheal status of the pigs had a significant influence on the occurrence of Giardia. Among the 14 Cryptosporidium and 96 Giardia isolates, two Cryptosporidium species (C. suis and C. scrofarum) and three G. duodenalis assemblages (A, B, and E) were found, among which assemblage A (56.25%) was the most prevalent. Additionally, 13 novel genotypic variations were identified at the bg, gdh, and tpi genes of G. duodenalis.

conclusionPigs in the CHT region harbor Cryptosporidium and Giardia with notable genotypic diversity and zoonotic potential. Addressing this issue requires further research within a One Health framework, focusing on integrated surveillance and the development of evidence-based policies for pig farming to reduce transmission and safeguard animal and public health.

Indexed as

CryptosporidiosisCryptosporidiumGiardiaGiardia lambliaGiardiasisSwine DiseasesAnimalsBangladeshDNA, ProtozoanFecesGenetic VariationGenotypePhylogenyPrevalenceProtozoan ProteinsSwineDNA, ProtozoanProtozoan ProteinsBangladeshChattogram Hill TractsCryptosporidium spp.Giardia duodenalisMolecular epidemiologyPigs

Identifiers

PMID41148460

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.