Evidence map›Paper›PMID 41148392›Full record

ArticleVeterinary research communications2025

Protective effect of selenomethionine on spleen and sacculus rotundus injury induced by aflatoxin B1 in reserve male rabbits.

Zhe Zhang, Shiyang Liu, Yumei Liu, Chaoying Zhang, Zihao Meng, Qiongxia Lv, Dongliu Luo, Xiaoguang Chen, Xuemin Zhu, Ziqiang Zhang

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Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhe ZhangCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
Shiyang LiuCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
Yumei LiuCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China. yumeiliu@haust.edu.cn.
Chaoying ZhangCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
Zihao MengCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
Qiongxia LvCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
Dongliu LuoCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
Xiaoguang ChenCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
Xuemin ZhuCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
Ziqiang ZhangCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China. ziqiangzhang@haust.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study is to explore the protective effect of selenomethionine (SeMet) against the immunotoxicity caused by aflatoxin B1 (AFB1) in male rabbits. Fifty healthy male rabbits aged 90 days were randomly divided into 5 groups: control group, model group, low dose selenomethionine group (Se-1), middle dose selenomethionine group (Se-2) and high dose selenomethionine group (Se-3). The control group and model group were fed basal diet, and the other three groups were fed SeMet 0.2 mg/kg, 0.4 mg/kg and 0.6 mg/kg respectively. On the 31st day of the experiment, All rabbits in the model group and three treatment groups were fed 0.5 mg/kg AFB1 for 21 days. Detect the levels of immune-related indicators in rabbit plasma. Collect samples, and observe the pathological changes in rabbit spleen and sacculus rotundus using hematoxylin-eosin (HE) staining. Analyze the expression of related proteins in spleen and sacculus rotundus tissues using immunofluorescence and Western blot (WB) analysis. Measure the levels of relevant inflammatory factor mRNA in spleen and sacculus rotundus tissues using qRT-PCR. Use enzyme-linked immunosorbent assay (ELISA) to detect the levels of oxidative stress-related indicators and inflammatory factors in spleen and sacculus rotundus tissues. The results indicate that AFB1 can induce oxidative stress and inflammation by activating the TLR-2/NF-κB signaling pathway, inhibiting the proliferation and differentiation of spleen and bursa cells, disrupting the normal structure of the spleen and sacculus rotundus tissues, and reducing the levels of immune-related indicators in plasma. SeMet pretreatment significantly inhibited the TLR-2/NF-κB signaling pathway, alleviating oxidative stress damage in spleen and sacculus rotundus tissues and reducing the inflammatory response in rabbits.Thus, we demonstrate that SeMet mitigates AFB1-induced immunotoxicity in reserve male rabbits by inhibiting the TLR-2/NF-κB signaling pathway. In addition, in this study, the effect of 0.4 mg/kg SeMet was the most effective. SeMet is a natural and safe drug that can prevent AFB1 poisoning in rabbit feed.

Indexed as

Aflatoxin B1Protective AgentsSelenomethionineSpleenAnimalsMaleRabbitsRandom AllocationAflatoxin B1Protective AgentsSelenomethionineAflatoxin B1Reserve male rabbitSacculus rotundusSelenomethionineSpleen

Identifiers

PMID41148392

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.