ArticleVeterinary ophthalmology2026
An Ex Vivo Morphometric Study of the Feline Corneal Endothelium (100 Eyes).
Article in Veterinary ophthalmology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
backgroundThe feline corneal endothelium plays a critical role in maintaining corneal clarity, yet little is known about its baseline morphology and age-related changes. This study aimed to assess endothelial morphology ex vivo using a vital dye-based imaging technique, offering a practical tool for assessing endothelial health in settings where in vivo imaging may be impractical or unavailable.
methodsCentral corneal buttons (8-mm) were collected post-mortem from 50 cats (100 eyes) and processed within 6 h of death or euthanasia. The endothelium was stained with 0.25% trypan blue and 0.5% alizarin red (pH 4.2) and imaged using light microscopy at 20× magnification. Morphometric analysis included mean cell density (MCD), mean cell area (MCA), polymegathism (coefficient of variation of cell area), and pleomorphism (% hexagonality). Statistical analyses assessed differences by age (< 10 vs. ≥ 10 years) and sex, with correlations to age explored via Spearman testing.
resultsMedian (±SEM; range) values across all eyes were as follows: MCA 352 ± 10 μm
conclusionsThis ex vivo approach enables detailed evaluation of feline corneal endothelial morphology, revealing clear age-related trends. The technique is practical and cost-effective for veterinary and translational research, with potential to support studies of endothelial health and dysfunction across species.
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