Evidence map›Paper›PMID 41147201›Full record

ArticleChembiochem : a European journal of chemical biology2025

Comparative Structural Analysis of Escherichia Coli Cyay at Room and Cryogenic Temperatures Using Macromolecular and Serial Crystallography.

Alaleh Shafiei, Nilufer Baldir, Jongbum Na, Jin Hae Kim, Hasan DeMirci

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Alaleh ShafieiDepartment of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey.ORCID https://orcid.org/0000-0003-2424-9583
Nilufer BaldirDepartment of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey.ORCID https://orcid.org/0000-0002-8829-3287
Jongbum NaDepartment of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, 42988, Republic of Korea.
Jin Hae KimDepartment of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, 42988, Republic of Korea.
Hasan DeMirciDepartment of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey.ORCID https://orcid.org/0000-0002-9135-5397

Funding

2232 International Fellowship for Outstanding Researchers Program of the TÜBİTAK 118C270National Research Foundation NRF-2021K2A9A1A06096295NSF Science and Technology Center NSF-1231306TÜBİTAK-KOREA(NRF) 2516 Bilateral Research Program 221N005
6 · The paper itself

Abstract

Frataxin is a 23 kDa mitochondrial iron-binding protein involved in the biogenesis of iron-sulfur (Fe-S) clusters. Deficiency in frataxin is associated with Friedreich's ataxia, a progressive neurodegenerative disorder. CyaY, the bacterial ortholog of eukaryotic frataxin, is believed to function as an iron donor in Fe-S cluster assembly, making it a key target for structural and functional studies. In this work, a comprehensive structural analysis of the Escherichia coli CyaY protein is presented, comparing its structure at room temperature and cryogenic conditions. Notably, the first room-temperature structures are obtained using the Turkish Light Source "Turkish DeLight" X-ray diffractometer and serial synchrotron X-ray crystallography, marking a significant step forward in understanding CyaY under near-physiological conditions.

Indexed as

Escherichia coliEscherichia coli ProteinsIron-Binding ProteinsCold TemperatureCrystallography, X-RayModels, MolecularProtein ConformationTemperatureEscherichia coli ProteinsIron-Binding ProteinscyaYfrataxinFriedreich ataxiaroom‐temperature crystallographystructural biology

Identifiers

PMID41147201
PMCPMC12582159

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.