Evidence map›Paper›PMID 41146334›Full record

ArticleBMC research notes2025

Influence of DEFB4 copy number variants on gene expression in monocytes.

Xianghong Zhang, Tingting Wen, Christoph Lippuner, Marcel Schiff, Frank Stuber

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Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Xianghong Zhang *Department of Cardiac Intensive Care Unit, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, Zhejiang, China. xianghong_zhang@zju.edu.cn.
Tingting Wen *Department of Anesthesiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Christoph LippunerDepartment of Anesthesiology and Pain Medicine, Bern University Hospital, Inselspital, Bern, Switzerland.
Marcel SchiffDepartment of Anesthesiology and Pain Medicine, Bern University Hospital, Inselspital, Bern, Switzerland.
Frank StuberDepartment of Anesthesiology and Pain Medicine, Bern University Hospital, Inselspital, Bern, Switzerland.

Funding

Wissenschaftlichen Fonds der Universitätsklinik für Anästhesiologie und Schmerztherapie Bern ZHXF-1-11
6 · The paper itself

Abstract

objectiveBeta-defensins, a group of antimicrobial peptides that can modulate immune response, are encoded by genes (DEFBs) on chromosome 8p23.1, a region subject to copy number variations (CNVs). This study aimed to investigate the influence of DEFB4 CNV on both mRNA and protein expression in human monocytes.

resultsA cohort of 862 healthy blood donors was screened for DEFB4 copy number (CN) by paralog ratio test and 66 individuals were recalled for a second blood donation. Monocytes were isolated from peripheral blood and stimulated by LPS. DEFB4 mRNA and β-defensin 2 protein levels were quantified using qPCR and ELISA, respectively. DEFB4 CN was precisely determined via ddPCR. DEFB4 mRNA was undetectable in unstimulated monocytes but inducible by LPS. Beta-defensin 2 was detectable in freshly isolated monocytes. After stimulation with LPS, beta-defensin 2 was strongly induced and the level was significantly higher than that of non-stimulated controls. A significant correlation between DEFB4 CN and mRNA expression level was found (P < 0.05, r

Indexed as

beta-DefensinsDNA Copy Number VariationsGene Expression RegulationMonocytesAdultFemaleGene ExpressionHumansLipopolysaccharidesMaleMiddle AgedRNA, Messengerbeta-DefensinsDEFB4A protein, humanLipopolysaccharidesRNA, MessengerBeta-defensin 2Copy number variationsDEFB4Gene expressionMonocyte

Identifiers

PMID41146334
PMCPMC12557836

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.