Evidence map›Paper›PMID 41146303›Full record

ArticleJournal of ovarian research2025

Quercetin-loaded mesenchymal stem cell derived extracellular vesicles enhance ovarian function in a cyclophosphamide induced ovarian damage.

Zeynep Ece Utkan Korun, Zehra Seda Halbutogullari, Yusufhan Yazir, Selenay Furat, Candan Altuntas, Ahmet Ozturk, Tugba Koldankaya, Serap Mert, Erkut Attar

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In one paragraph

Article in Journal of ovarian research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zeynep Ece Utkan KorunDepartment of Obstetrics and Gynecology, Faculty of Medicine, Yeditepe University, Istanbul, Türkiye.ORCID http://orcid.org/0000-0002-1595-569X
Zehra Seda HalbutogullariDepartment of Stem Cell and Tissue Regeneration, Institute of Health Sciences, Kocaeli University, Kocaeli, Türkiye. sedahalbutogullari@kocaeli.edu.tr.ORCID http://orcid.org/0000-0001-6810-4939
Yusufhan YazirDepartment of Stem Cell and Tissue Regeneration, Institute of Health Sciences, Kocaeli University, Kocaeli, Türkiye.ORCID http://orcid.org/0000-0002-8472-0261
Selenay FuratDepartment of Stem Cell and Tissue Regeneration, Institute of Health Sciences, Kocaeli University, Kocaeli, Türkiye.ORCID http://orcid.org/0000-0002-0501-1679
Candan AltuntasDepartment of Stem Cell and Tissue Regeneration, Institute of Health Sciences, Kocaeli University, Kocaeli, Türkiye.ORCID http://orcid.org/0000-0002-2551-1849
Ahmet OzturkDepartment of Stem Cell and Tissue Regeneration, Institute of Health Sciences, Kocaeli University, Kocaeli, Türkiye.ORCID http://orcid.org/0000-0001-8723-9417
Tugba KoldankayaDepartment of Polymer Science and Technology, Kocaeli University, Kocaeli, Türkiye.ORCID http://orcid.org/0000-0002-4557-2905
Serap MertCenter for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Umuttepe West Campus Research Building for Health Sciences, Department of Stem Cell F3, Izmit, Kocaeli, 41001, Türkiye.ORCID http://orcid.org/0000-0001-5939-5295
Erkut AttarDepartment of Obstetrics and Gynecology, Faculty of Medicine, Yeditepe University, Istanbul, Türkiye.ORCID http://orcid.org/0000-0002-8459-2538

Funding

Scientific Research Projects Coordination Unit of Kocaeli University TDK-2021-2626
6 · The paper itself

Abstract

backgroundTo investigate whether intraovarian administration of quercetin-loaded extracellular vesicles derived from Wharton's jelly mesenchymal stem cells (EVs-QUE) improves ovarian function in a cyclophosphamide (CTX)-induced premature ovarian insufficiency (POI) rat model.

methodsHuman Wharton's jelly-derived mesenchymal stem cells (MSCs) were cultured, and extracellular vesicles (EVs) were isolated and characterized by flow cytometry and electron microscopy. Quercetin was loaded onto EVs using ultrasonic incubation to generate EVs-QUE. A rat model of CTX-induced POI was established, and the subjects received intraovarian injections of either EVs or EVs-QUE. Ovarian function was assessed through histological evaluation, immunofluorescence staining, and gene expression analysis.

resultsTreatment with EVs-QUE significantly improved ovarian morphology and folliculogenesis, reduced the number of atretic follicles, and decreased Casp3 expression. Proliferation markers (Ki67, Pcna) and antioxidant genes (Nrf2, Sod1) were upregulated. Additionally, steroidogenesis- and oocyte-related genes (Star, Gdf9, Bmp15) showed increased expression. Although systemic hormonal alterations were limited, local tissue analysis confirmed a regenerative effect in the EVs-QUE group.

conclusionsQuercetin-loaded EVs derived from Wharton's jelly MSCs enhanced ovarian recovery in a CTX-induced POI model through anti-apoptotic, pro-proliferative, and antioxidant pathways. These findings suggest that intraovarian administration of EVs-QUE may represent a promising strategy for fertility preservation and warrant further investigation in long-term and translational studies.

Indexed as

CyclophosphamideExtracellular VesiclesMesenchymal Stem CellsOvaryPrimary Ovarian InsufficiencyQuercetinAnimalsDisease Models, AnimalFemaleHumansRatsWharton JellyCyclophosphamideQuercetinFolliculogenesisMesenchymal stem cell-derived extracellular vesiclesOxidative stressPremature ovarian failureQuercetinWharton’s jelly

Identifiers

PMID41146303
PMCPMC12557886

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.