ArticleVirology journal2025
Giant grouper nervous necrosis virus subgenomic RNA3 is transcribed by a premature termination mechanism via intragenomic RNA-RNA pairing.
Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- The multifaceted challenge of viral nervous necrosis in fish farming: a review of current knowledge and control.Veterinary research communications · 2026Review
- Translation of nervous necrosis virus involves eIF4E but not RPS6 phosphorylation and viral particle assembly in remodeled microtubule-organizing center.Virology journal · 2025Article
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNervous necrosis virus (NNV) is a bipartite positive-sense RNA virus that causes viral nervous necrosis disease in larval and juvenile marine fish. In its genome, the RNA1 segment encodes RNA-dependent RNA polymerase (RdRp) for viral RNA synthesis, while RNA2 encodes coat protein for viral particle assembly. In addition, an RNA1-derived subgenomic RNA3 region encodes the non-structural B2 protein, which antagonizes host RNA interference mechanisms. However, the mechanisms of RNA3 transcription remain unclear.
methodsA DNA-based reverse genetic platform vector (pCMV-DRz) for recombinant (r) giant grouper (GG) NNV recovery was constructed. End sequences of GGNNV RNAs were determined by 5' rapid amplification of cDNA ends (RACE) and/or circular RNA sequencing approach. Replication and transcription of viral RNAs in GGNNV-infected or mutant clones transfected grouper brain (GB) cells were detected by Northern blotting using anti-sense RNA DIG-labeling probes. Recombinant viral proteins were detected by using Western blotting or immunocytochemistry staining after GB cells transfected with pCMV-DRz-RNA1 or infected with rGGNNV. In addition, the viral kinetics of rGGNNV was determined using TCID
resultsIn the reverse genetic platform, high viral titer of rGGNNV (10
conclusionOur results suggest that NNV utilizes premature termination mechanism for subgenome RNA3 transcription and NNV RNAs expression is coordinately regulated by viral cis- and trans-RNA sequences.
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