ArticleVeterinary clinical pathology2025
Electrophoretic Plasma Protein Reference Intervals for Backyard Chickens From Northern Colorado.
Article in Veterinary clinical pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Comparison of capillary zone and agarose gel protein electrophoresis in macaws (Frontiers in veterinary science · 2026Article
- Electrophoretic Plasma Protein Reference Intervals for Backyard Chickens From Northern Colorado.Veterinary clinical pathology · 2025Article
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Authors and funding
4 authors.
Funding
Abstract
backgroundThe number of backyard chicken (Gallus gallus domesticus) flocks in the US in urban and suburban areas has continued to increase over the past three decades. These flocks are often raised as both production animals and as pets. Electrophoretic evaluation of plasma proteins has been held as the reference standard for protein evaluation. Reference intervals (RIs) for plasma protein electrophoresis (PPE) in chickens in northern Colorado are not readily available but could aid diagnostic evaluations.
objectivesWe aimed to generate RIs for PPE in adult non-molting backyard chicken flocks in northern Colorado.
methodsPlasma from 120 healthy adult chickens in 7 flocks located in northern Colorado was used. Electrophoretic evaluation was completed using a biuret total protein assay and agarose gel electrophoresis, with proteins fractionated into 6 parts. RIs for PPE fractions were generated, and partitioning by sex was performed according to ASVCP guidelines.
resultsRIs were generated for all birds and after partitioning by sex. For all birds, agarose gel electrophoretic RIs were: A:G 0.24-1.20, albumin 1.33-2.91 g/dL, alpha-1 globulin 0.13-0.47 g/dL, alpha-2 globulin 0.33-0.91 g/dL, beta-1 globulin 0.24-1.50 g/dL, beta-2 globulin 0.33-1.79 g/dL, and gamma globulin 0.26-0.82 g/dL. A statistical difference, p < 0.05, was noted between hens and roosters for all electrophoretic fractions except total alpha globulin, beta-2 globulin, and A:G, and partitioning was warranted based on the method recommended by Lahti; the data were partitioned by sex, and RIs were generated.
conclusionsRIs are available for PPE in backyard chickens in northern Colorado.
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