ArticleJournal of visualized experiments : JoVE2025
A Cost-Effective and Minimally Invasive Protocol for Chronic Multi-Site Electroencephalography Recording in Freely Moving Mice.
Article in Journal of visualized experiments : JoVE, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Simplified method for EEG recordings in mice.MethodsX · 2026Article
- A minimally invasive EEG recording method in mice using thin needle electrodes.bioRxiv : the preprint server for biology · 2026Article
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Authors and funding
6 authors.
Funding
Abstract
Electroencephalography (EEG) serves as a fundamental tool for tracking electrical activity in the brain and has become increasingly important in both clinical diagnostics and preclinical animal research. While rodent EEG offers a powerful platform for modeling human neurological disorders, long-term recordings are often limited by the high cost, invasiveness, or technical complexity of existing systems. Here, we present a reliable, accessible, economically viable, and minimally intrusive protocol for chronic, multi-site cortical EEG acquisition in freely moving mice. The technique involves fabricating platinum-iridium wires with screw electrodes connected to a universal serial bus (USB)-type interface, which are anchored epidurally using bone screws and secured with adhesive and dental cement. The system allows for stable signal capture over several months, as demonstrated in wild-type C57BL/6J mice with an 85% post-operative survival rate (n = 20). EEG was sampled at 1,000 Hz and filtered between 1 Hz and 500 Hz using a differential amplifier setup. To validate the system, pentylenetetrazol (20 mg/kg) was administered to induce seizure-like activity, leading to a marked increase in interictal discharges-from 2-5 at baseline to 20-51 events post-injection-accompanied by increased signal amplitude. Discharges were identified based on waveform morphology and signal characteristics matching recognized electrophysiological criteria. The setup is compatible with behavioral video tracking and open-source analysis pipelines, and supports integration with pharmacological, genetic, or neuromodulation studies. This method provides a stable and adaptable platform for investigating brain network dynamics in rodent models, with applications in epilepsy research and beyond.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.