Evidence map›Paper›PMID 41144216›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Two- and Three-Dimensional Human Intestinal Tissue Models for Cryptosporidium Infection.

Daviel Cardenas, Seema Bhalchandra, Hymlaire Lamisere, Honorine D Ward

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Daviel Cardenas *Tufts Medical Center, Boston, MA, USA.
Seema Bhalchandra *Tufts Medical Center, Boston, MA, USA.
Hymlaire Lamisere *Tufts Medical Center, Boston, MA, USA.
Honorine D WardTufts Medical Center, Boston, MA, USA. honorine.ward@tuftsmedicine.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conventional cell cultures utilizing transformed or immortalized cell lines or primary human epithelial cells have played a fundamental role in furthering our understanding of Cryptosporidium infection. However, they remain inadequate with respect to their inability to emulate in vivo conditions and support long-term growth and completion of the parasite's life cycle. Previously, we developed a three-dimensional (3D) silk scaffold-based model using transformed human intestinal epithelial cells (IECs). This model supported C. parvum infection for up to 2 weeks and resulted in completion of the life cycle of the parasite. However, transformed IECs are not representative of primary human IECs.Human intestinal organoids (hIOs) or human intestinal enteroids (hIEs) are 3D cultures derived from Lgr5

Indexed as

CryptosporidiosisCryptosporidiumCryptosporidium parvumIntestinal MucosaOrganoidsCell Culture TechniquesEpithelial CellsHumansTissue ScaffoldsDrug screenEnteroidIntestinalMonolayerOrganoidPermeable supportSilk scaffoldStem cellThree dimensional (3D)TissueTranswellTwo dimensional (2D)

Identifiers

PMID41144216

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.