Evidence map›Paper›PMID 41144214›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Establishing TurboID Proximity Labeling and mAID Conditional Knockdown System in Cryptosporidium parvum.

Rui Xu, L David Sibley

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Rui XuState Key Laboratory for Animal Disease Control and Prevention Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
L David SibleyDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA. sibley@wustl.edu.

Funding

Genetic Basis of Host Infectivity by CryptosporidiumR01AI175150 · NIAID · WASHINGTON UNIVERSITY · PI L. David Sibley · 2024 to 2026
$2.0M
Influence of microbial metabolites on mitosome function in CryptosporidiumR01AI192672 · NIAID · WASHINGTON UNIVERSITY · PI L. David Sibley · 2025 to 2026
$1.4M
NIAID NIH HHS R01 AI175150NIAID NIH HHS R01 AI192672
6 · The paper itself

Abstract

Protein interaction networks and subcellular proteomes can provide key insights into the biological functions of protein complexes. Here, we describe a method for proximity-based labeling using TurboID to define protein interaction networks in Cryptosporidium parvum. In addition, we have developed an auxin-inducible degron (AID) labeling system for conditional protein degradation in C. parvum to facilitate functional studies. This chapter describes the procedures for generating miniTurbo or mAID-TIR1-tagged C. parvum strains and validating the TurboID and mAID systems for target genes.

Indexed as

Cryptosporidium parvumGene Knockdown TechniquesProtein Interaction MappingProtozoan ProteinsStaining and LabelingIndoleacetic AcidsProtein Interaction MapsIndoleacetic AcidsProtozoan ProteinsConditional knockdownCRISPR/Cas9DegronMass spectrometryProximity labeling

Identifiers

PMID41144214

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.