Evidence map›Paper›PMID 41144197›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Cryptosporidium Diagnostic Assays: Molecular Detection and Species Determination by Real-Time PCR-Based Assays.

Guy Robinson, Kristin Elwin, Rachel M Chalmers

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Recurrent zoonoticEpidemiology and infection · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Guy RobinsonCryptosporidium Reference Unit, Public Health Wales Microbiology and Health Protection, Singleton Hospital, Swansea, Wales, UK. Guy.Robinson@wales.nhs.uk.
Kristin ElwinCryptosporidium Reference Unit, Public Health Wales Microbiology and Health Protection, Singleton Hospital, Swansea, Wales, UK.
Rachel M ChalmersCryptosporidium Reference Unit, Public Health Wales Microbiology and Health Protection, Singleton Hospital, Swansea, Wales, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular diagnostic assays for Cryptosporidium are usually based on polymerase chain reaction (PCR) and may detect the entire genus or target-specified species. Of the ever-growing number of species, fewer than half have been reported from humans, and most human cases of cryptosporidiosis are caused by Cryptosporidium parvum or Cryptosporidium hominis. Here, we describe a real-time PCR for the detection of all Cryptosporidium spp. that can then be differentiated by sequencing the PCR amplicons and a duplex real-time PCR for the simultaneous detection and differentiation of C. parvum and C. hominis.

Indexed as

CryptosporidiosisCryptosporidiumMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionDNA, ProtozoanFecesHumansDNA, ProtozoanC. hominisC. parvumCryptosporidiumDetectionDNA extractionReal-time PCR

Identifiers

PMID41144197

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.