Evidence map›Paper›PMID 41143997›Full record

ArticleApplied microbiology and biotechnology2025

Impact of Clostridium butyricum HADIG-CB003 dietary supplementation on the gut microbiota of Kunming mice.

Yilin Pang, Xianxian Xu, Daizhi Li, Yue Ge, Jiamin Liang, Xiaojun Xiong, Shaohuai Wen, Xiaokai Li, Yueqiu Wang, Xiongying Li and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yilin Pang *College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China.
Xianxian Xu *Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Daizhi LiZhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Yue GeZhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Jiamin LiangHunan Agricultural Development & Investment Group Company, Hunan Animal Pharmaceutical Company, Hunan New Wellful Company Limited, 28 Weiluoke Road, Changsha, China.
Xiaojun XiongHunan Agricultural Development & Investment Group Company, Hunan Animal Pharmaceutical Company, Hunan New Wellful Company Limited, 28 Weiluoke Road, Changsha, China.
Shaohuai WenHunan Agricultural Development & Investment Group Company, Hunan Animal Pharmaceutical Company, Hunan New Wellful Company Limited, 28 Weiluoke Road, Changsha, China.
Xiaokai LiXiangyang City Huancheng Park Management Office, Xiangcheng District, Xiangyang, Hubei, China.
Yueqiu WangHunan Agricultural Development & Investment Group Company, Hunan Animal Pharmaceutical Company, Hunan New Wellful Company Limited, 28 Weiluoke Road, Changsha, China.
Xiongying LiHunan Agricultural Development & Investment Group Company, Hunan Animal Pharmaceutical Company, Hunan New Wellful Company Limited, 28 Weiluoke Road, Changsha, China.
Guoqiang TanZhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China. tgq@wmu.edu.cn.
Yuanshan LinCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China. linyuanshan@hunau.net.ORCID http://orcid.org/0000-0002-1637-8353

Funding

Innovation Platform Open Fund Project of Colleges and Universities of Hunan Province 19k045Key Discipline of Zhejiang Province in Medical Technology category AKey Discipline of Zhejiang Province in Medical Technology first classThe double first-class construction project of Hunan Agricultural University SYL201802003
6 · The paper itself

Abstract

Clostridium butyricum is a key anaerobic probiotic due to its ability to produce butyric acid and form spores. In this study, four strains were isolated from pig feces using tryptose sulfite cycloserine (TSC) plates and specific primers sequentially. The optimal strain, C. butyricum HADIG-CB003, identified via gas chromatography and enzyme tests, produced 1.54 g/L of butyric acid, with cellulase and amylase activities of 20.88 ± 1.27 U/mL and 16.04 ± 0.46 U/mL, respectively. In reinforced clostridial medium, it produced 2.48 L of gas per liter, with hydrogen making up 59.68% (1.48 L). The adhesion rate to porcine intestinal epithelial cells (IEC-J2) was 9.16% in vitro. Twelve 3-week-old male Kunming mice were split into two groups and fed a basal diet with or without 1 × 10

Indexed as

Clostridium butyricumDietary SupplementsGastrointestinal MicrobiomeProbioticsAnimalsAnimals, Outbred StrainsBacterial AdhesionButyric AcidFecesMaleMiceRNA, Ribosomal, 16SSwineButyric AcidRNA, Ribosomal, 16SAmylaseButyric acidClostridium butyricumGut microbiotaProbiotic

Identifiers

PMID41143997
PMCPMC12559120

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.