Evidence map›Paper›PMID 41143911›Full record

ArticleMetabolic brain disease2025

Inhibition of ventral tegmental area projections to the paraventricular thalamus attenuates conditioned cues-induced heroin seeking behavior in rats.

Yiying Zhou, Wenchang Li, Dandan Cai, Dingding Zhuang, Xin Zhang, Miaojun Lai, Weisheng Chen, Wenhua Zhou, Huaqiang Zhu

Abstract read
PubMed Publisher
In one paragraph

Article in Metabolic brain disease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yiying ZhouZhejiang Provincial Key Lab of Addiction Research, The Affiliated Kangning Hospital of Ningbo University, Ningbo, 315201, China.
Wenchang LiZhejiang Pharmaceutical University, Zhejiang Province, Ningbo, 315199, China.
Dandan CaiZhejiang Provincial Key Lab of Addiction Research, The Affiliated Kangning Hospital of Ningbo University, Ningbo, 315201, China.
Dingding ZhuangZhejiang Provincial Key Lab of Addiction Research, The Affiliated Kangning Hospital of Ningbo University, Ningbo, 315201, China.
Xin ZhangZhejiang Provincial Key Lab of Addiction Research, The Affiliated Kangning Hospital of Ningbo University, Ningbo, 315201, China.
Miaojun LaiZhejiang Provincial Key Lab of Addiction Research, The Affiliated Kangning Hospital of Ningbo University, Ningbo, 315201, China.
Weisheng ChenZhejiang Provincial Key Lab of Addiction Research, The Affiliated Kangning Hospital of Ningbo University, Ningbo, 315201, China.
Wenhua ZhouZhejiang Provincial Key Lab of Addiction Research, The Affiliated Kangning Hospital of Ningbo University, Ningbo, 315201, China. whzhou@vip.163.com.
Huaqiang ZhuZhejiang Pharmaceutical University, Zhejiang Province, Ningbo, 315199, China. zhuhq@zjpu.edu.cn.ORCID 0000-0001-5275-2106

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ventral tegmental area (VTA) is typically involved in heroin reward, but it is still unclear whether the VTA or neural projections containing VTA are also involved in conditioned cues (CS)-induced heroin-seeking behavior. In present study, rats were first trained to self-administer heroin for 14 days, then undergone withdrawal for another 14 days. Subsequently, part of rats were assessed CS-induced heroin-seeking behaviors and tested c-Fos protein expression in brain areas after microinjection with saline, neostigmine or tetrodotoxin (TTX) into the VTA. Part of rats were tested CS-induced heroin-seeking behaviors after chemogenetic inhibition of the dopaminergic projection from VTA to paraventricular thalamus (PVT) by stimulating with clozapine N-oxide (CNO). We found that CS-induced heroin-seeking behaviors could be enhanced by microinjection with neostigmine, but weakened by microinjection with TTX, into the VTA. The expression of c-Fos immunoreactive neurons were increased in the PVT and nucleus accumbens (NAc) after neostigmine treatment, but decreased in the PVT, NAc and lateral habenula (LHb) after TTX treatment. Chemogenetic inhibition of the dopaminergic projection from VTA to PVT could significantly inhibit CS-induced heroin-seeking behaviors of rats. Our results suggest that stimulating VTA promotes CS-induced heroin-seeking behavior, and the underlying neural circuits may involve the projection from VTA to PVT.

Indexed as

CuesDrug-Seeking BehaviorHeroinMidline Thalamic NucleiVentral Tegmental AreaAnimalsClozapineMaleNeostigmineNeural PathwaysProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleySelf AdministrationTetrodotoxinClozapineHeroinNeostigmineProto-Oncogene Proteins c-fosTetrodotoxinChemogeneticConditioned cuesHeroin seekingNeostigmineParaventricular nucleusVentral tegmental area

Identifiers

PMID41143911

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.