Evidence map›Paper›PMID 41143062›Full record

ArticleClinical & translational immunology2025

A semi-automated ASC speck assay to evaluate pyrin inflammasome activation.

Pei Dai, Oliver Skinner, Xufeng Lin, Aiden Telfser, Stephanie Ruiz-Diaz, Rohit G Saldanha, Katie Frith, Ming-Wei Lin, Kahn Preece, Paul E Gray and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical & translational immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Pei DaiPrecision Immunology Program Garvan Institute of Medical Research Sydney NSW Australia.ORCID https://orcid.org/0000-0002-9022-5101
Oliver SkinnerImmune Biotherapies Program Garvan Institute of Medical Research Sydney NSW Australia.
Xufeng LinComputational Biology Group, Data Science Platform Garvan Institute of Medical Research Sydney NSW Australia.
Aiden TelfserPrecision Immunology Program Garvan Institute of Medical Research Sydney NSW Australia.
Stephanie Ruiz-DiazPrecision Immunology Program Garvan Institute of Medical Research Sydney NSW Australia.
Rohit G SaldanhaDepartment of General Paediatrics Sydney Children's Hospital Sydney NSW Australia.
Katie FrithRandwick Clinical Campus, School of Clinical Medicine, Faculty of Medicine and Health UNSW Sydney Sydney NSW Australia.
Ming-Wei LinDepartment of Clinical Immunology Westmead Hospital Sydney NSW Australia.
Kahn PreecePaediatric Immunology Department John Hunter Children's Hospital Newcastle NSW Australia.
Paul E GrayDepartment of Immunology Sydney Children's Hospital Sydney NSW Australia.
Alberto Pinzon-CharryQueensland Children's Hospital Brisbane QLD Australia.
Anna SullivanQueensland Children's Hospital Brisbane QLD Australia.
Stephen AdelsteinDepartment of Clinical Immunology and Allergy and Institute of Academic Medicine Royal Prince Alfred Hospital Sydney NSW Australia.
Winnie Wy TongSt Vincent's Healthcare Clinical Campus, School of Clinical Medicine, Faculty of Medicine and Health UNSW Sydney Sydney NSW Australia.
Matthew Js ParkerCentral Clinical School, Faculty of Medicine and Health University of Sydney Sydney NSW Australia.
Laila GirgisSt Vincent's Healthcare Clinical Campus, School of Clinical Medicine, Faculty of Medicine and Health UNSW Sydney Sydney NSW Australia.
Brynn WainsteinDepartment of Paediatrics Bankstown-Lidcombe Hospital Sydney NSW Australia.
Samar OjamiMonash Pathology, Monash Health Melbourne VIC Australia.
Elissa K DeenickPrecision Immunology Program Garvan Institute of Medical Research Sydney NSW Australia.
Leonard D GoldsteinSt Vincent's Healthcare Clinical Campus, School of Clinical Medicine, Faculty of Medicine and Health UNSW Sydney Sydney NSW Australia.
Michael J RogersSt Vincent's Healthcare Clinical Campus, School of Clinical Medicine, Faculty of Medicine and Health UNSW Sydney Sydney NSW Australia.
Tri Giang PhanPrecision Immunology Program Garvan Institute of Medical Research Sydney NSW Australia.ORCID https://orcid.org/0000-0002-4909-2984

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To develop a rapid functional assay to validate variants of uncertain significance (VUS) in the Methods: Overactivity of the pyrin inflammasome pathway and ASC speck oligomerisation in response to stimulation with low concentrations of Results: The semi-automated ASC speck assay is able to discriminate between healthy controls and patients with familial Mediterranean fever (FMF) and pyrin inflammasome overactivity with high sensitivity. It is also able to discriminate pyrin inflammasome overactivity from other autoinflammatory disease controls with high specificity. Conclusion: The semi-automated ASC speck assay may be a useful test to functionally validate VUS in the

Indexed as

ASC speckfunctional testinginflammasomeMEFVpyrinVUS

Identifiers

PMID41143062
PMCPMC12549213

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.