Evidence map›Paper›PMID 41141380›Full record

ArticleThe breast journal2025

MicroRNA-195-5p Targets MYB to Regulate Proliferation and Malignant Metastasis in Triple-Negative Breast Cancer via PI3K/AKT/mTOR Signaling.

Kewei Tang, Site Bai, Qiang Zhou, Songlian Liu, Leilan Yin, Yajun Tong, Ling Long, Ludi Ou, Qinghua Yin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The breast journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kewei TangDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0003-6995-5163
Site BaiDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0001-3966-1944
Qiang ZhouDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0001-9138-9888
Songlian LiuDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0009-3337-8193
Leilan YinDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0003-1358-851X
Yajun TongDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0005-9047-4828
Ling LongDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0007-9466-9539
Ludi OuDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0003-2164-6484
Qinghua YinDepartment of Oncology, Yueyang Central Hospital, Yueyang, Hunan, China.ORCID 0009-0007-6721-5937

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To investigate the effect of microRNA-195-5p (miRNA-195-5p) on proliferation and malignant metastasis in triple-negative breast cancer (TNBC) cells and its underlying mechanism. Methods: Expression levels of miRNA-195-5p and MYB were determined by quantitative real-time PCR (RT-qPCR) in TNBC cells (MDA-MB-231 and BT-549) and normal human mammary epithelial cells (MCF-10A). Cell proliferation was assessed via CCK-8 assays after miRNA-195-5p overexpression or knockdown in MDA-MB-231 cells. Transwell assays evaluated cellular invasion and migration. Western blotting analyzed impacts on the PI3K/AKT/mTOR pathway. Targeting of MYB by miRNA-195-5p was confirmed using TargetScan prediction and dual-luciferase reporter assays. RT-qPCR measured MYB expression upon miRNA-195-5p modulation. Rescue experiments (co-overexpression of MYB and miRNA-195-5p) further assessed proliferation and PI3K/AKT/mTOR signaling via CCK-8 and Western blotting. Results: Compared to MCF-10A cells, miRNA-195-5p expression was significantly downregulated ( Conclusion: miRNA-195-5p suppresses proliferation and metastasis in TNBC by targeting MYB and inhibiting the PI3K/AKT/mTOR pathway.

Indexed as

MicroRNAsProto-Oncogene Proteins c-mybTriple Negative Breast NeoplasmsCell Line, TumorCell MovementCell ProliferationFemaleGene Expression Regulation, NeoplasticHumansNeoplasm MetastasisPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionTOR Serine-Threonine KinasesMicroRNAsMIRN195 microRNA, humanMTOR protein, humanMYB protein, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-mybTOR Serine-Threonine KinasesMicroRNA-195-5pMYBPI3K/AKT/mTOR signalingproliferationtriple-negative breast cancer

Identifiers

PMID41141380
PMCPMC12549211

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.