Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes.

Tsugumi Yamaji, Yuko Katayama, Nanase Arata, Mariko Sasaki

Abstract read

In one paragraph

Article in FEBS letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tsugumi YamajiLaboratory of Gene Quantity Biology, Center for Frontier Research, National Institute of Genetics, Mishima, Shizuoka, Japan.

Yuko KatayamaLaboratory of Gene Quantity Biology, Center for Frontier Research, National Institute of Genetics, Mishima, Shizuoka, Japan.

Nanase ArataLaboratory of Gene Quantity Biology, Center for Frontier Research, National Institute of Genetics, Mishima, Shizuoka, Japan.

Mariko SasakiLaboratory of Gene Quantity Biology, Center for Frontier Research, National Institute of Genetics, Mishima, Shizuoka, Japan.ORCID https://orcid.org/0000-0003-4208-1251

Funding

Fusion Oriented REsearch for disruptive Science and Technology JPMJFR214PJapan Society for the Promotion of Science 24K09417Research Organization of Information and SystemsTakeda Science Foundation

6 · The paper itself

Abstract

DNA copy number changes are the most frequent genomic alterations in cancer cells. Here, we demonstrate that Rad27/FEN1, a structure-specific nuclease in budding yeast, plays a crucial role in maintaining the stability of the ribosomal DNA (rDNA) repeats. Severe rDNA instability is observed in the rad27∆ mutant, independently of Fob1-mediated DNA replication fork arrest and DNA double-strand break (DSB) formation in the rDNA. The rad27Δ mutant accumulates Okazaki fragments in the rDNA region, without inducing the formation of detectable DSBs. Similar rDNA instability is observed in DNA ligase

Indexed as

DNADNA Copy Number VariationsDNA, RibosomalFlap EndonucleasesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsDNA-Binding ProteinsDNA Breaks, Double-StrandedDNA ReplicationExodeoxyribonucleasesProliferating Cell Nuclear AntigenDNADNA-Binding ProteinsDNA, Ribosomalexodeoxyribonuclease IExodeoxyribonucleasesFlap EndonucleasesFOB1 protein, S cerevisiaeOkazaki fragmentsProliferating Cell Nuclear AntigenRAD27 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsDNA copy number changesgenome instabilityOkazaki fragmentRad27rDNA instabilitysingle‐strand break

Identifiers

PMID41140146

DOI10.1002/1873-3468.70193

PMCPMC12720220

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.