Evidence map›Paper›PMID 41140082›Full record

ArticleProtein and peptide letters2025

Shepherin II Gene Synthesis and Peptide Characterization:

Azza Abd Elfattah, Safia Samir, Hend Okasha, Azza Ahmed Atef, Alshaimaa Taha

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Article in Protein and peptide letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Azza Abd ElfattahDepartment of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
Safia SamirDepartment of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza 12411, Egypt.ORCID 0000-0002-4121-0448
Hend OkashaDepartment of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza 12411, Egypt.ORCID 0000-0003-1125-245X
Azza Ahmed AtefDepartment of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
Alshaimaa TahaDepartment of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.ORCID 0000-0002-6825-466X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThe shepherin II peptide is characterized by a histidine/glycine-rich sequence. This study aimed to design, express recombinantly, and evaluate the antiviral activity of shepherin II against hepatitis A virus (HAV).

methodsThe shepherin II gene was reverse-translated, cloned into the pET-3a vector, and expressed in

resultsThe mass spectrometry confirmed a primary peptide fragment with a molecular weight of 3,421.30 Da (100% relative abundance). SDS-PAGE verified peptide expression. Cytotoxicity tests on Vero cells showed a CC DISCUSSION: The recombinant expression of shepherin II was successfully achieved and confirmed by mass spectrometry and SDS-PAGE. The peptide showed measurable antiviral activity against HAV.

conclusionThis study demonstrated the feasibility of recombinant shepherin II production and assessed its antiviral activity. However, the limited selectivity index of shepherin II remains a challenge that needs to be addressed through molecular modification or alternative delivery strategies to improve its clinical potential.

Indexed as

Antiviral AgentsEscherichia coliHepatitis A virusPeptidesAnimalsChlorocebus aethiopsGene ExpressionRecombinant ProteinsVero CellsAntiviral AgentsPeptidesRecombinant Proteinsantimicrobial peptideantiviral activityHAV.Histidine-glycine rich peptiderecombinant DNA technologyshepherin peptides

Identifiers

PMID41140082

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.