Evidence map›Paper›PMID 41138186›Full record

ArticleCell reports2025

Chromatin remodeling activity of EP400 safeguards chromosomal stability by preventing CENP-A mislocalization.

Subhash Chandra Sethi, Roshan L Shrestha, Vinutha Balachandra, Tetsuya Hori, Luca Corda, Shinjen Lin, Laurent Ozbun, Tatiana S Karpova, Katherine McKinnon, Raj Chari and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Subhash Chandra SethiGenetics Branch, National Cancer Institute (NCI), NIH, Bethesda, MD, USA.
Roshan L ShresthaGenetics Branch, National Cancer Institute (NCI), NIH, Bethesda, MD, USA; Developmental Therapeutics Branch, NCI, NIH, Bethesda, MD, USA.
Vinutha BalachandraGenetics Branch, National Cancer Institute (NCI), NIH, Bethesda, MD, USA.
Tetsuya HoriGraduate School of Frontier Biosciences, The University of Osaka, Suita, Osaka, Japan.
Luca CordaLaboratory of Genome Evolution, Sapienza University of Rome, Rome, Italy.
Shinjen LinFunctional Genomics Laboratory, NCATS, NIH, Bethesda, MD, USA.
Laurent OzbunLaboratory of Receptor Biology and Gene Expression, NCI, NIH, Bethesda, MD, USA.
Tatiana S KarpovaLaboratory of Receptor Biology and Gene Expression, NCI, NIH, Bethesda, MD, USA.
Katherine McKinnonCCR Vaccine Branch Flow Cytometry Core, NCI, NIH, Bethesda, MD, USA.
Raj ChariGenome Modification Core (GMC), Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Gianluca PegoraroLaboratory of Receptor Biology and Gene Expression, NCI, NIH, Bethesda, MD, USA.
Ken Chih-Chien ChengFunctional Genomics Laboratory, NCATS, NIH, Bethesda, MD, USA.
Simona GiuntaLaboratory of Genome Evolution, Sapienza University of Rome, Rome, Italy.
Natasha J CaplenGenetics Branch, National Cancer Institute (NCI), NIH, Bethesda, MD, USA.
Tatsuo FukagawaGraduate School of Frontier Biosciences, The University of Osaka, Suita, Osaka, Japan.
Munira A BasraiGenetics Branch, National Cancer Institute (NCI), NIH, Bethesda, MD, USA. Electronic address: basraim@nih.gov.

Funding

Molecular Determinants of Chromosome Transmission and Cell Cycle RegulationZIABC010822 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI BASRAI, MUNIRA · 2009 to 2025
$29.2M
HiTIF Microscopy Core FacilityZICBC011567 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI PEGORARO, GIANLUCA · 2014 to 2025
$15.8M
Discovery of genes required for expression or activity of fusion oncogenesZIABC011704 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI CAPLEN, NATASHA · 2016 to 2025
$15.8M
LRBGE Optical Microscopy CoreZICBC011574 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI KARPOVA, TATIANA · 2014 to 2025
$7.4M
Molecular Determinants of Chromosome Transmission and Cell Cycle RegulationZ01BC010822 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI BASRAI, MUNIRA · 2007 to 2008
$1.9M
Intramural NIH HHS Z01 BC010822Intramural NIH HHS Z99 CA999999Intramural NIH HHS ZIA BC010822Intramural NIH HHS ZIA BC011704Intramural NIH HHS ZIC BC011567Intramural NIH HHS ZIC BC011574NCI NIH HHS HHSN261201500003CNCI NIH HHS HHSN261201500003I
6 · The paper itself

Abstract

The mislocalization of CENP-A to non-centromeric regions contributes to chromosomal instability (CIN). The NuA4 histone acetyltransferase complex members EP400 and KAT5 regulate histone H2A.Z-H2B exchange and acetylation of histones, respectively. Overexpression of CENP-A and mutations in NuA4 components are observed in cancers. Here, we define a role for the chromatin remodeling activity of EP400, a top hit in RNAi screens for increased nuclear levels of CENP-A, in preventing CENP-A mislocalization and CIN. Mechanistically, we demonstrate a defect in the extraction of CENP-A from chromatin in cells expressing the EP400

Indexed as

AutoantigensCentromere Protein AChromatin Assembly and DisassemblyChromosomal InstabilityChromosomal Proteins, Non-HistoneHistone AcetyltransferasesCell LineChromatinHistonesHumansTumor Suppressor Protein p53AutoantigensCENPA protein, humanCentromere Protein AChromatinChromosomal Proteins, Non-HistoneHistone AcetyltransferasesHistonesTumor Suppressor Protein p53ATPase activityCENP-Acentromerechromatin remodelerchromosomal instabilityCP: Molecular biologyEP400histone acetylationKAT5kinetochoreNuA4 HAT complex

Identifiers

PMID41138186
PMCPMC12746837

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.