Evidence map›Paper›PMID 41136966›Full record

ArticleBMC oral health2025

Decoding the human tongue of light chain amyloidosis in three dimensions (3D): three-dimensional pathological visualization drives early and precise screening of suspected cases.

Liqiong Wu, Fanyuan Sun, Si Xu, Xiaoli Gao, Jing Chen, Zheng Zhang, Zhiqiang Liu, Yongcun Qu, Zuomin Wang

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In one paragraph

Article in BMC oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Liqiong Wu *Department of Stomatology, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongti South Road, Beijing, 100020, China.
Fanyuan Sun *Capital University of Physical Education and Sports, Beijing, 100191, China.
Si XuDepartment of Stomatology, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongti South Road, Beijing, 100020, China.
Xiaoli GaoDepartment of Stomatology, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongti South Road, Beijing, 100020, China.
Jing ChenCapital University of Physical Education and Sports, Beijing, 100191, China.
Zheng ZhangDepartment of Stomatology, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongti South Road, Beijing, 100020, China.
Zhiqiang LiuDepartment of Stomatology, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongti South Road, Beijing, 100020, China.
Yongcun QuCapital University of Physical Education and Sports, Beijing, 100191, China. quyongcun@cupes.edu.cn.
Zuomin WangDepartment of Stomatology, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongti South Road, Beijing, 100020, China. wzuomin@sina.cn.

Funding

Zuomin Wang Grant No. L232104
6 · The paper itself

Abstract

objectivesThis study aimed to apply CUBIC tissue clearing combined with three-dimensional (3D) imaging to visualize the pathological architecture of systemic light chain (AL) amyloidosis in the human tongue, assess spatial and microstructural changes associated with amyloid deposition, and explore its potential to enhance early diagnostic accuracy. MATERIALS AND

methodsSeven tongue specimens from confirmed AL amyloidosis cases underwent CUBIC processing, multiplex immunofluorescence staining, and 3D imaging. Vascular density, surface area, vessel and muscle fiber diameters were compared between amyloid deposition and non-deposition regions. Ten additional specimens initially negative for AL amyloidosis were re-evaluated using the same procedure, suspected cases were verified by repeat histology and clinical follow-up.

resultsIn amyloid deposition regions, vascular density and surface area were (0.14 ± 0.04) μm

conclusionsCUBIC-based 3D imaging enables detailed visualization and quantification of amyloid-associated microstructural changes in tongue tissue, providing deeper insight into disease mechanisms and serving as a valuable complement to conventional histopathology for enhancing early diagnostic accuracy in AL amyloidosis.

Indexed as

AmyloidosisImaging, Three-DimensionalImmunoglobulin Light-chain AmyloidosisTongueAgedEarly DiagnosisFemaleHumansMaleMiddle AgedAmyloidosis ALEarly diagnosisHistopathologyThree-dimensional imagingTissue clearingTongue

Identifiers

PMID41136966
PMCPMC12551279

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