ArticleBMC genomics2025
Identification and functional analysis of circular RNAs during mitochondrial damage induced by infectious bovine rhinotracheitis virus infection in Madin-Darby bovine kidney cells.
Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Chlorophyllide a Oxygenase (CAO) Gene Duplication Across the Viridiplantae.Journal of molecular evolution · 2025Article
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Authors and funding
14 authors.
Funding
Abstract
backgroundInfectious bovine rhinotracheitis virus (IBRV), a member of the Herpesviridae family, causes infectious bovine rhinotracheitis (IBR) and induces mitochondrial dysfunction in host cells. Circular RNAs (circRNAs)-a novel class of non-coding RNAs-have been implicated in various biological processes and pathologies related to mitochondrial damage. However, their role in IBRV-induced mitochondrial damage in Madin-Darby bovine kidney (MDBK) cells remains unclear.
resultsTransmission electron microscopy(TEM), laser confocal microscopy, and flow cytometry confirmed that IBRV infection causes mitochondrial damage in MDBK cells. High-throughput sequencing revealed 144 differentially expressed (DE) circRNAs, 725 messenger RNAs (mRNAs), and 160 microRNAs (miRNAs) in IBRV-infected cells. We predicted that DE circRNAs regulate mitochondrial damage via source genes of circRNA, circRNA-miRNA-mRNA networks, and RNA-binding proteins (RBPs). Source genes of circRNA were enriched in mitochondria-related pathways, such as the mammalian target of rapamycin (mTOR), thyroid hormone, and Hippo signalling; 11 genes were localized to mitochondria. CircRNA-miRNA-mRNA network target genes were associated with cellular senescence, mitophagy, and ubiquitin-mediated proteolysis; 471 genes were linked to mitochondria. Additionally, 961 RBPs were enriched in pathways, such as nucleocytoplasmic transport and RNA degradation; 107 RBPs were localized to mitochondria. Functional validation revealed knockdown of circ_002584 reduced reactive oxygen species (ROS) accumulation (p < 0.05) and mitochondrial membrane potential depolarization (p < 0.05). Knockdown of circ_004326 increased both (p < 0.01).
conclusionsCircRNAs play a regulatory role in IBRV-induced mitochondrial damage within MDBK cells. This finding is significant for virus-associated mitochondrial damage research, forming a theoretical foundation for utilizing circRNAs as diagnostic biomarkers and potential therapeutic targets for IBR.
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