ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2025
Isolation and characterization of lytic bacteriophages against Staphylococcus aureus.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Progress of Bacteriophage Research and Application in the Treatment of Bovine Mastitis: A Review.Veterinary sciences · 2026Review
- A comprehensive analysis of the kinetics of infection of lytic bacteriophages specific to the ESKAPE and critical pathogens.World journal of microbiology & biotechnology · 2026Review
- ThreeViruses · 2026Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study was conceived with the objective to isolate and characterize lytic bacteriophages (phages) against Staphylococcus aureus (S. aureus) associated with bovine mastitis. Phages were isolated from enriched slurry and milk samples using the agar overlay technique. Characterization included plaque morphology, Field Emission Scanning Electron Microscopy (FE-SEM), pH and thermal stability, host range (spot assay), adsorption assay and one-step growth curve analysis. Lytic efficacy was assessed using the Efficiency of Plating (EOP) method. Five phages (pS1 to pS5) were isolated, of which pS4 and pS5 were temperate phages as indicated by its lysis pattern. The analysis showed that phages pS2 and pS3 produced clear, pinpoint-sized plaques while pS1 formed large, clear plaques. FE-SEM classified all three phages under the class Caudoviricetes. Adsorption assay and one step growth curve analysis revealed that the phages had an adsorption time ranging from 9 to 15 min, and a latent period between 30 and 50 min and burst sizes of 68 (pS2), 220 (pS1) and 280 (pS3) pfu/cell. Stability tests revealed significant difference in thermal stability at 60 °C and 70 °C (p < 0.0001), and in pH tolerance from pH 2 to 10 (p < 0.0001) among pS1, pS2 and pS3. The spot assay revealed that the complete panel of clinical S. aureus isolates (n = 18), standard cultures (ATCC 12600 and ATCC 29213) and clinical isolates of S. chromogenes and S. haemolyticus were sensitive to pS1. In contrast, pS2 and pS3 exhibited lytic activity exclusively against the S. aureus isolates, showing no activity against S. chromogenes or S. haemolyticus. Phage pS1 demonstrated good lytic effect against 10/21 test isolates compared to pS2 (3/21) and pS3 (3/21). Overall, the study characterized three lytic phages targeting S. aureus implicated in bovine mastitis and showed its in vitro efficacy against planktonic S. aureus.
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