Evidence map›Paper›PMID 41136665›Full record

ArticleCurrent microbiology2025

Molecular Evidence of Chlorotic Stunt Disease Induced by Mungbean yellow mosaic India virus (MYMIV) in Lentil (Lens esculentus).

Mohammad Ansar, Vikash Kumar, Tushar Ranjan, Mohammad Akram, Jitendra Nath Srivastava, Rashid Mumtaz Khan, Md Arshad Anwer, Ravi Ranjan Kumar, Mohammad Feza Ahmad, Arun Prasad Bhagat

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mohammad AnsarDepartment of Plant Pathology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813 210, India. ansar.pantversity@gmail.com.ORCID http://orcid.org/0000-0002-3852-741X
Vikash KumarDepartment of Plant Pathology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813 210, India.
Tushar RanjanDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813 210, India.
Mohammad AkramDivision of Crop Protection, ICAR-Indian Institute of Pulses Research, Kanpur, U.P., 208 024, India.
Jitendra Nath SrivastavaDepartment of Plant Pathology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813 210, India.
Rashid Mumtaz KhanDepartment of Chemistry, College of Science, Qassim University, Buraidah, 51452, Qassim, Saudi Arabia.
Md Arshad AnwerDepartment of Plant Pathology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813 210, India.
Ravi Ranjan KumarDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813 210, India.
Mohammad Feza AhmadDirectorate of Seed and Farm, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813 210, India.
Arun Prasad BhagatDepartment of Plant Pathology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813 210, India.

Funding

Anusandhan National Research Foundation (Formerly Science and Engineering Research Board), Department of Science and Technology, Govt. of India CRG/2020/003111
6 · The paper itself

Abstract

Diseases caused by Begomovirus are a severe obstacle for dicotyledonous plants worldwide. In Bihar, a northern Indian state, we have been seeing indicators of stunting and chlorosis in lentil plants, with an incidence of 2.90%. Based on the series of tests between 2021 and 2024, the suspected organism was determined to be Begomovirus. After restriction digestion, HindIII and BglI, the RCA of three DNA samples revealed a linearized DNA of about ~ 2.7 kb. With a genomic organization consisting of two ORFs in virion-sense and five ORFs in complementary sense separated by IR region, the associated virus was discovered to have 2746 nucleotide for DNA-A. When compared to the isolates of Mungbean yellow mosaic India virus (MYMIV), the genome sequences of DNA-A and DNA-B exhibited the highest nucleotide identity (98%). A single recombination event has been detected in DNA-A, with the major and minor parent classified as MYMIV isolates from Bangladesh and Paskistan, respectively. Two overlapping primers were designed using nucleotide sequence of MYMIV-Lentil isolate in order to amplify the virus's genome. DNA-A and DNA-B showed PCR-mediated amplification rates of 70.8 and 69.6%, respectively, when both primer sets were used. In order to ensure linked virus, whitefly-mediated transmission was tried in a controlled environment. In five examined lentil types, a meagre transmission rate (6.1-7.8%) was observed. The present evidence that a virus infects lentil crop rather than other microbe that cause stunting offers valuable insight for developing effective management measures.

Indexed as

BegomovirusLens PlantPlant DiseasesDNA, ViralGenome, ViralIndiaOpen Reading FramesPhylogenySequence Analysis, DNADNA, Viral

Identifiers

PMID41136665

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.