Evidence map›Paper›PMID 41134884›Full record

ArticleScience advances2025

Enhancing cross-organelle coordination to advance plant cytochrome P450 functionality in yeast.

Shanhui Xu, Songbo Wei, Yun Xiong, Xiaoxuan Teng, Kyle Sipe, Alair Abad, Theo Poucet, Yinsheng Wang, Yanran Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shanhui XuAiiso Yufeng Li Family Department of Chemical and Nano, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.ORCID 0009-0009-5466-7904
Songbo WeiDepartment of Chemistry, University of California, Riverside, Riverside, CA 92521, USA.ORCID 0000-0001-9311-1823
Yun XiongDepartment of Chemistry, University of California, Riverside, Riverside, CA 92521, USA.
Xiaoxuan TengAiiso Yufeng Li Family Department of Chemical and Nano, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.ORCID 0000-0001-9568-027X
Kyle SipeAiiso Yufeng Li Family Department of Chemical and Nano, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Alair AbadAiiso Yufeng Li Family Department of Chemical and Nano, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.ORCID 0009-0003-1067-2310
Theo PoucetFrench National Research Institute for Agriculture, Food and Environment, University of Bordeaux, Biology of Fruit and Pathogens Laboratory, UMR 1332, F-33140 Villenave d'Ornon, France.ORCID 0000-0001-8910-9245
Yinsheng WangDepartment of Chemistry, University of California, Riverside, Riverside, CA 92521, USA.ORCID 0000-0001-5565-283X
Yanran LiAiiso Yufeng Li Family Department of Chemical and Nano, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.ORCID 0000-0001-8709-3497

Funding

Chemical Biology of DNA and RNA AlkylationR35ES031707 · NIEHS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Yinsheng Wang · 2020 to 2026
$6.1M
Research Training in Environmental ToxicologyT32ES018827 · NIEHS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Yinsheng Wang · 2010 to 2026
$5.3M
Reprogramming Plant Perception for Novel Plant Natural Products DiscoveryDP2AT011445 · NCCIH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LI, YANRAN · 2020 to 2020
$2.3M
NCCIH NIH HHS DP2 AT011445NIEHS NIH HHS R35 ES031707NIEHS NIH HHS T32 ES018827
6 · The paper itself

Abstract

Sustainable production of plant secondary metabolites remains a substantial challenge. Microbial cell factories offer a promising solution, yet reconstituting plant cytochrome P450s, key enzymes in these pathways, remains a major hurdle. In this study, we investigated a plant membrane protein, AtMSBP1, for its ability to broadly enhance P450 functionality in engineered yeast. Unlike in plants, interactions with cytochrome P450s are not essential for AtMSBP1's function in yeast. Specifically, AtMSBP1 orchestrates interorganelle communication, involving not only the endoplasmic reticulum but also mitochondria and vacuoles, supporting cytochrome P450s functionality in yeast. This study highlights the critical role of cross-organelle coordination in supporting cytochrome P450s functionality, emphasizing a shift from traditional ER-based approaches to engineering cross-organelle interactions and tailoring the cellular microenvironment to enhance plant cytochrome P450s functionality in yeast.

Indexed as

Arabidopsis ProteinsCytochrome P-450 Enzyme SystemPlant ProteinsSaccharomyces cerevisiaeEndoplasmic ReticulumMitochondriaVacuolesArabidopsis ProteinsCytochrome P-450 Enzyme SystemPlant Proteins

Identifiers

PMID41134884
PMCPMC12551693

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.