Evidence map›Paper›PMID 41134300›Full record

ArticleBiomacromolecules2025

Evaluation of Peptide Nucleic Acid Encapsulation in Ferritin Nanocages for Gene Silencing Applications.

Andrea Patrizia Falanga, Maria Vittoria Farina, Gabriele Cianfoni, Lorenzo Barolo, Chiara Di Meo, Giulia Elizabeth Borsatti, Francesca Ghirga, Bruno Botta, Luca Pisano, Nicola Borbone and 5 more

Abstract read
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In one paragraph

Article in Biomacromolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Andrea Patrizia FalangaDepartment of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, Naples 80131, Italy.
Maria Vittoria FarinaDepartment of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy.
Gabriele CianfoniDepartment of Chemistry and Technology of Drugs, Sapienza-University of Rome, Rome 00185, Italy.ORCID 0000-0002-6969-2071
Lorenzo BaroloDepartment of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy.
Chiara Di MeoDepartment of Chemistry and Technology of Drugs, Sapienza-University of Rome, Rome 00185, Italy.ORCID 0000-0002-3531-6107
Giulia Elizabeth BorsattiDepartment of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy.
Francesca GhirgaDepartment of Chemistry and Technology of Drugs, Sapienza-University of Rome, Rome 00185, Italy.ORCID 0000-0002-5591-5190
Bruno BottaDepartment of Chemistry and Technology of Drugs, Sapienza-University of Rome, Rome 00185, Italy.ORCID 0000-0001-8707-4333
Luca PisanoDepartment of Chemistry and Technology of Drugs, Sapienza-University of Rome, Rome 00185, Italy.
Nicola BorboneDepartment of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, Naples 80131, Italy.
Stefano D'ErricoDepartment of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, Naples 80131, Italy.
Alessio PaoneDepartment of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy.
Giorgia OlivieroDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Sergio Pansini 5, Naples 80131, Italy.ORCID 0000-0003-0765-2127
Deborah QuaglioDepartment of Chemistry and Technology of Drugs, Sapienza-University of Rome, Rome 00185, Italy.ORCID 0000-0001-7762-8283
Paola BaioccoDepartment of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, Rome 00185, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Peptide nucleic acids (PNAs) feature a neutral peptide-like backbone, providing nuclease resistance and potential for precision medicine and diagnostics through specific DNA and RNA binding. However, their therapeutic use is hindered by poor solubility and cell permeability. In this study, we demonstrated that negatively charged PNAs can be readily loaded into the polycationic Humanized Archaeoglobus Ferritin, namely, PA3.2-HumAfFt bioconjugate system, following a divalent-cation-triggered oligomerization technique. The versatility of PNA chemistry enabled the production of synthetic nucleic acid homologues with varying lengths and charges, ranging from positive to negative. We evaluated the loading performance of HumAfFt with and without chemical modifications and investigated the release dynamics of PNAs under conditions simulating the intracellular environment. Our findings demonstrated the effective uptake, release, and biological activity of PNAs in cancer cells, notably silencing the GAPDH gene with good efficiency. This evaluation paves the way for optimizing PNA-based therapeutics and broadening their applications.

Indexed as

FerritinsGene SilencingPeptide Nucleic AcidsHumansFerritinsPeptide Nucleic Acids

Identifiers

PMID41134300
PMCPMC12606638

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.