Evidence map›Paper›PMID 41133888›Full record

ArticleEpigenomics2025

Expression of G-quadruplex coordinates BRCA1, CDH1, and RASSF1 via DNA methylation in mouse breast cancer cells.

Xinchen Zhou, Zhuo Jin, Xintong Zhan, Shuhui Yang, Yang Jiang, Helin Wang, Zhuo Zhang, Shuhan Dong, Wei Xia, Limei Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xinchen ZhouCollege of Medical Technology, Beihua University, Jilin, China.ORCID 0000-0002-7597-7604
Zhuo JinCollege of Medical Technology, Beihua University, Jilin, China.
Xintong ZhanCollege of Medical Technology, Beihua University, Jilin, China.
Shuhui YangCollege of Medical Technology, Beihua University, Jilin, China.
Yang JiangCollege of Medical Technology, Beihua University, Jilin, China.
Helin WangCollege of Medical Technology, Beihua University, Jilin, China.
Zhuo ZhangCollege of Medical Technology, Beihua University, Jilin, China.
Shuhan DongCollege of Medical Technology, Beihua University, Jilin, China.
Wei XiaCollege of Medical Technology, Beihua University, Jilin, China.
Limei LiuCollege of Medical Technology, Beihua University, Jilin, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundG-quadruplex (G4) structures are enriched in key genomic regions and regulate gene expression and chromosomal stability. However, their role in de novo DNA methylation remains unclear. DNA methyltransferase 3A (DNMT3A) and DNA methyltransferase 3B (DNMT3B) are vital for cancer initiation and progression. This study investigated the role of G4 structures in regulating DNMT3A and DNMT3B expression and their epigenetic function in breast cancer.

methodsG4 structures in DNMT3A and DNMT3B were identified using Quadruplex forming G-Rich Sequences Mapper, circular dichroism, and gel mobility shift assays. The effects of the G4 stabilizer pyridostatin on breast cancer 4T1 cell proliferation, migration, DNMT3A and DNMT3B expression, and promoter methylation of Breast Cancer 1 (BRCA1), E-cadherin (CDH1), and Ras Association Domain Family Member 1 (RASSF1) were evaluated.

resultsG4-forming sequences were found in the core promoter regions of DNMT3A and DNMT3B. They formed parallel G4 structures in vitro. Pyridostatin enhanced G4 stability, inhibited 4T1 cell proliferation and migration, downregulated DNMT3A and DNMT3B expression, reduced promoter methylation of BRCA1 and RASSF1, and altered target gene expression.

conclusionPromoter G4 structures actively regulate gene expression by modulating de novo DNA methylation, suggesting that targeting G4s may represent a novel therapeutic strategy for breast cancer.

Indexed as

BRCA1 ProteinBreast NeoplasmsCadherinsDNA MethylationG-QuadruplexesTumor Suppressor ProteinsAnimalsCell Line, TumorCell MovementCell ProliferationDNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3ADNA Methyltransferase 3BFemaleGene Expression Regulation, NeoplasticHumansBRCA1 ProteinCadherinsDNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3ADNA Methyltransferase 3BDnmt3a protein, mouseTumor Suppressor ProteinsBreast cancerDNA methylationDNA methyltransferaseepigenetic regulationgene regulationG-quadruplex

Identifiers

PMID41133888
PMCPMC12826751

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