Evidence map›Paper›PMID 41133884›Full record

ArticleThe Biochemical journal2025

Kinetic investigation of calcium-induced Sorcin aggregation by stopped-flow light scattering.

Qiushi Ye, Kathleen Joyce Carillo, Nicolas Delaeter, Lei Zhang, Jaekyun Jeon, Yanxin Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Biochemical journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Qiushi YeSchool of Physics, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.ORCID 0000-0002-5569-1545
Kathleen Joyce CarilloInstitute for Bioscience and Biotechnology Research, University of Maryland | National Institute of Standard and Technology, Rockville, MD, 20850, U.S.A.
Nicolas DelaeterInstitute for Bioscience and Biotechnology Research, University of Maryland | National Institute of Standard and Technology, Rockville, MD, 20850, U.S.A.
Lei ZhangSchool of Physics, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.ORCID 0000-0002-4880-824X
Jaekyun JeonInstitute for Bioscience and Biotechnology Research, University of Maryland | National Institute of Standard and Technology, Rockville, MD, 20850, U.S.A.
Yanxin LiuInstitute for Bioscience and Biotechnology Research, University of Maryland | National Institute of Standard and Technology, Rockville, MD, 20850, U.S.A.ORCID 0000-0002-2253-3698

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sorcin, a penta-EF hand calcium-binding protein, is implicated in multidrug resistance (MDR) in various cancers and has roles in neurodegenerative diseases. It regulates cellular calcium homeostasis by interacting with calcium channels, pumps, and exchangers in a calcium-dependent manner. Calcium binding induces a conformational change in Sorcin, exposing hydrophobic surfaces that mediate protein interactions and calcium flux between the cytosol and endoplasmic reticulum. These exposed surfaces can also drive Sorcin aggregation in the absence of binding partners. Here, we exploited calcium-induced conformational changes and aggregation of Sorcin as a model to study its calcium sensitivity and aggregation mechanisms. Stopped-flow light scattering revealed that Sorcin aggregation is reversible, co-operative, and primarily influenced by Sorcin concentration rather than physiological calcium levels. Our findings suggest that the calcium sensitivity of Sorcin is finely tuned by its expression level, highlighting its role as an intracellular calcium sensor. This work establishes Sorcin as a model system for studying protein aggregation mechanisms with implications for MDR and neurodegenerative diseases.

Indexed as

CalciumCalcium-Binding ProteinsProtein AggregatesHumansKineticsProtein ConformationScattering, RadiationCalciumCalcium-Binding ProteinsProtein AggregatesSRI protein, humancalcium binding proteinmultidrug resistanceprotein aggregationSorcinstopped-flow

Identifiers

PMID41133884
PMCPMC12687447

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.