Evidence map›Paper›PMID 41133461›Full record

ArticlePlant biotechnology journal2026

The PtrC2H2.2-6-PtrCYP86A7/A8 Module Regulates Poplar Drought Tolerance Through Mediating Cutin and Wax Biosynthesis Pathways.

Jiu-Jiu Zhao, Shuang-Lian Deng, Hao Li, Shu-Ying Wei, Rui-Quan Wang, Yuan Zhang, Xiang Xiang, Peng Yang, Jing Li, Ting Wang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jiu-Jiu ZhaoForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.ORCID https://orcid.org/0009-0009-6852-3258
Shuang-Lian DengForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Hao LiForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Shu-Ying WeiForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Rui-Quan WangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Yuan ZhangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Xiang XiangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Peng YangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Jing LiForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Ting WangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Jinliang HuangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Liang-Hua ChenForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
HanBo YangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Xue-Qin WanForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.ORCID https://orcid.org/0000-0002-9252-1625
Fang HeForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station, College of Forestry, Sichuan Agricultural University, Chengdu, China.ORCID https://orcid.org/0000-0003-4078-5650

Funding

National Natural Science Foundation of China 32101481National Natural Science Foundation of China 32471820Sichuan Province Science and Technology Support Program 2024YFNH0028
6 · The paper itself

Abstract

The plant cuticle, composed of cutin and wax, is crucial for reducing non-stomatal water loss and enhancing drought tolerance. CYP86A genes are key regulators of cutin and wax biosynthesis, yet the mechanisms controlling their expression remain unclear. Here, we identified PtrC2H2.2-6, a C2H2-type transcription factor that is downregulated at both transcriptional and protein levels under water stress in poplar. Through bioinformatics and biochemical analyses, we demonstrated that PtrC2H2.2-6 interacts with PtrPPK1 to regulate drought tolerance by modulating two critical cuticle biosynthesis genes, PtrCYP86A7 and PtrCYP86A8. PtrC2H2.2-6 negatively regulates cutin and wax biosynthesis by binding to the CACT motif in the promoters of PtrCYP86A7 and PtrCYP86A8, thereby suppressing their expression. Conversely, PtrPPK1 interacts with PtrC2H2.2-6, phosphorylates it, and promotes its degradation, thereby relieving its repression of PtrCYP86A7/8. Functional validation revealed that RNAi-mediated silencing of PtrC2H2.2-6 enhanced wax accumulation and drought tolerance, while overexpression of PtrC2H2.2-6 reduced wax accumulation and drought resistance. Furthermore, overexpression of PtrCYP86A7 or PtrCYP86A8 increased wax accumulation, enhanced the water retention capacity of the leaf surface, and improved drought resistance. Collectively, the PtrPPK1-PtrC2H2.2-6-PtrCYP86A7/A8 module is a critical regulatory mechanism for drought tolerance in poplar, offering potential targets for breeding drought-resistant forest trees.

Indexed as

Membrane LipidsPlant ProteinsPopulusTranscription FactorsWaxesDrought ResistanceDroughtsGene Expression Regulation, PlantcutinMembrane LipidsPlant ProteinsTranscription FactorsWaxesdroughtPopulusPtrC2H2.2‐6PtrCYP86A7PtrCYP86A8

Identifiers

PMID41133461
PMCPMC12946459

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.