Evidence map›Paper›PMID 41132936›Full record

ArticleFrontiers in plant science2025

PacBio single-molecule long-read transcriptome sequencing and analysis of somatic embryogenesis in

Jinling Dai, Shengli Zhang, Yu'e Bai

Abstract read
In one paragraph

Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jinling DaiCollege of Forestry, Inner Mongolia Agricultural University, Hohhot, China.
Shengli ZhangCollege of Forestry, Inner Mongolia Agricultural University, Hohhot, China.
Yu'e BaiCollege of Forestry, Inner Mongolia Agricultural University, Hohhot, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: We performed long-read transcriptome sequencing of pooled samples from various somatic embryogenesis stages using PacBio SMRT technology. The obtained transcripts were functionally annotated using the Nr, SwissProt, KEGG, and KOG databases. We conducted comprehensive transcript structure analyses, including identification of alternative splicing, SSR loci, lncRNAs, and transcription factors. Furthermore, we cloned the Results: Our analysis generated 12,232 high-quality transcripts. We identified 83 genes with alternative splicing, 1,006 SSR loci, 35 lncRNAs, and 548 transcription factors from 46 distinct families. The Discussion: This study establishes the first reference-quality transcriptome database for

Indexed as

BABY BOOMlong-read transcriptomePicea mongolicasomatic embryogenesistranscription factors

Identifiers

PMID41132936
PMCPMC12540384

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.