Evidence map›Paper›PMID 41132916›Full record

ArticleApplications in plant sciences

From WGS to gels: Development and testing of PCR primers targeting toxic

Elizabeth Sage Hunter, Sydnee Fo, Robert Literman, Richard H Uva, Jennifer L Wolny, Sara M Handy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Elizabeth Sage HunterU.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition Office of Regulatory Science 5001 Campus Dr. College Park 20740 Maryland USA.ORCID https://orcid.org/0000-0002-5628-1281
Sydnee FoU.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition Office of Regulatory Science 5001 Campus Dr. College Park 20740 Maryland USA.
Robert LitermanU.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition Office of Analytics and Outreach 5001 Campus Dr. College Park 20740 Maryland USA.ORCID https://orcid.org/0000-0002-1784-0407
Richard H UvaSeaberry Farm 2770 Wright Rd. Federalsburg 21632 Maryland USA.
Jennifer L WolnyU.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition Office of Regulatory Science 5001 Campus Dr. College Park 20740 Maryland USA.ORCID https://orcid.org/0000-0002-3556-5015
Sara M HandyU.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition Office of Regulatory Science 5001 Campus Dr. College Park 20740 Maryland USA.ORCID https://orcid.org/0000-0003-3861-4609

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Premise: This study capitalized on a library of single-nucleotide polymorphisms created via whole genome sequencing (WGS) to develop and test a PCR assay for detecting toxic Methods: Ten pairs of PCR primers were designed, optimized, and tested against a subset of vouchered specimens. Two primer sets were screened using 55 vouchered Plantaginaceae species, complex food matrices, five different plant tissues, a dilution series, and spiked food products with 0.5%, 1%, and 5% biomass of Results: At optimized annealing temperatures, these primers amplified only Discussion: This study provides an enhanced DNA-based method for detecting

Indexed as

dietary supplementsDigitalisfoodsfoxglovegenomicsPCRtoxic plants

Identifiers

PMID41132916
PMCPMC12542805

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.