Evidence map›Paper›PMID 41132896›Full record

ArticleEngineering in life sciences2025

In Vivo Online Monitoring of Intracellular Lipid Accumulation in

Kira Müntjes, Lesley Plücker, Magnus Philipp, Paul Richter, Marcel Mann, Michael Feldbrügge, Kerstin Schipper

Abstract read
In one paragraph

Article in Engineering in life sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kira MüntjesInstitute of Microbiology Heinrich-Heine University Düsseldorf Düsseldorf Germany.
Lesley PlückerInstitute of Microbiology Heinrich-Heine University Düsseldorf Düsseldorf Germany.
Magnus PhilippInstitute of Microbiology Heinrich-Heine University Düsseldorf Düsseldorf Germany.
Paul RichterBioeconomy Science Center (BioSC) C/O Forschungszentrum Jülich Jülich Germany.
Marcel MannBioeconomy Science Center (BioSC) C/O Forschungszentrum Jülich Jülich Germany.
Michael FeldbrüggeInstitute of Microbiology Heinrich-Heine University Düsseldorf Düsseldorf Germany.
Kerstin SchipperInstitute of Microbiology Heinrich-Heine University Düsseldorf Düsseldorf Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single cell oils produced in microorganisms constitute appealing alternatives to plant oils. Oleaginous fungi accumulate triacylglycerols in lipid droplets (LD). Their biosynthesis is typically induced under nitrogen limitation. We exploit the fungal model Summary: Microbial oils are promising, environmentally friendly alternatives to plant oils and have the potential of a huge market share once competitive production and isolation processes are accomplished.Online monitoring is key to efficient engineering of single cell oil producing microorganisms and bioprocess optimization in order to achieve competitive products.Here, we present an inexpensive, fluorescence-based reporter that can be used to track the approximate oil accumulation of microbial cultures in vivo. This omits the use of expensive dyes or offline methodology with a high workload.While we established the biosensor in the yeast form of the fungal microorganism

Indexed as

fluorescence reporterlipid dropletsonline monitoringsingle‐cell oilUstilago maydis

Identifiers

PMID41132896
PMCPMC12541552

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.